水稻野生型愈伤组织中耐盐细胞频率的影响因素

利用组织培养技术研究愈伤组织的倍性、品种、外植体对野生型愈伤组织中耐盐细胞频率的影响。发现 :( 1)单倍体和二倍体愈伤组织的耐盐细胞频率比较接近 ,四倍体愈伤组织的耐盐细胞频率一般比单倍体和二倍体愈伤组织的小 ;( 2 )不同品种的耐盐细胞频率差异大 ;( 3)二倍体植株幼穗培养产生的二倍体愈伤组织和四倍体植株花药培养产生的二倍体愈伤组织的耐盐细胞频率没有显著的差异

Factors Affecting the Rate of Salt Tolerant Cell in the Callus Wild Type of Rice(Oryza sativa L.)

The effects of callus ploidy level,variety,explanton the rate of the salttolerant cell in the wild type callus of rice(Oryza sativa L.) were investigated in the present experiment. It was found that:(1 ) the wild type haploid and diploid calluses showed no significant difference in terms of the rate of salt tolerant cell,while the autotetraploid callus had lower rate of the salt tolerant cell than its respective haploid and diploid calli;(2 ) the variety had significant effect on the rate of salttolerantcell;(3) there were no significantdifference in terms of the rate of the salt tolerant cell between the wild type diploid callus derived from the young panicle culture of the diploid plants and the wild type diploid callus derived from the anther culture of autotetraploid plants.