纤维素同步糖化发酵生产乙醇

[目的]利用微生物方法生产乙醇，从而替代化石能源。[方法}土曲霉M11利用纤维素为原料产酶并糖化纤维素成还原糖，利用酿酒酵母发酵生成乙醇。，[结果]通过对土曲霉M11生长条件的研究，确定了土曲霉M11的最佳培养时间是3d，最佳接种量为200μl，最适培养湿度为80％，最适培养温度为45℃，最适培养pH为3．0，此条件下可获得最大的产酶量。通过对糖化过程的研究，确定了纤维素酶的最适糖化温度为55℃，最适pH为5．0，此条件下可获得较高的还原糖量，且在酸性条件下酶活力较高，具有很好的热稳定性。通过发酵，还原糖量占原材料干重的62．42％，产生的乙醇占原材料干重的21．36％。[结论]此方法可以应用于工业发酵生产乙醇，有利于保护环、降低成本、提高社会效益，有很好的应用价值。

Research on the Ethanol Produced from the Simultaneous Glycation and Fermentation of Cellulose

[Objective] The ethanol was produced by means of microbiological processes for the replacing approach of energy source.[Method] The reducing sugar was produced from the cellulose,which was saccharified by the enzyme that was from the cellulose as raw material was acted by Aspergillus terreus-M11,and the ethanol was produced based on the fermentation of yeast.[Result] The experimental result indicated that the optimal culture condition of Aspergillus terreus-M11 growth was that the best time was 3 days,the b...