| 淫羊藿植物DNA指纹图谱的构建 |
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| 引用本文： | 李明军,杜明凤.淫羊藿植物DNA指纹图谱的构建[J].安徽农业科学,2011,39(18):10820-10824. |
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| 作者姓名： | 李明军 杜明凤 |
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| 作者单位： | 贵州省林业调查规划院,贵州,贵阳,550003;贵州师范大学,贵州,贵阳,550001 |
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| 摘 要： | 目的]建立淫羊藿植物的DNA指纹图谱。方法]对来自贵州、四川等的17种淫羊藿植物进行RAPD标记研究。结果]从80个引物q-筛选出13个谱带清晰、多态性好的引物，共扩增得到263条谱带，多态性条带259条，多态性百分率为98．5％，建立了17种淫羊藿植物的DNA指纹图谱。结论]每个物种均有各自特异的共有谱带、特有谱带、缺失谱带，这些谱带可用于淫羊藿的物种鉴定。
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| 关 键 词： | 淫羊藿 RAPD DNA指纹图谱 鉴定 |
Establishment of DNA Fingerprints on Epimedium L. |
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| LI Ming-jun et al.Establishment of DNA Fingerprints on Epimedium L.[J].Journal of Anhui Agricultural Sciences,2011,39(18):10820-10824. |
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| Authors: | LI Ming-jun |
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| Institution: | LI Ming-jun et al(Guizhou Province Forestry Survey and Planning Institute,Guiyang,Guizhou 550003) |
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| Abstract: | Objective] The aim was to establish the DNA fingerprinting on Epimedium L..Method] 17 species of Epimedium natived to Guizhou and Sichuan etc.were studied by means of RAPD.Result] From 80 primers,13 primers were selected which could amplify clear and reproducible polymorphic bands.The PCR results showed that a total of 263 bands were amplified,of which 259 bands were polymorphic.Interestingly,the average rate of polymorphic bands was 98.5%,which were selected for the construction of DNA fingerprinting.C... |
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| Keywords: | Epimedium RAPD DNA Fingerprint Identification |
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