| 乐清市铁皮石斛总黄酮含量的测定 |
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| 引用本文： | 吴佳雯,鲍佳路,吕耀平,钟仙龙,项丽,徐安利. 乐清市铁皮石斛总黄酮含量的测定[J]. 安徽农业科学, 2011, 39(18): 10856+10884-10856,10884 |
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| 作者姓名： | 吴佳雯 鲍佳路 吕耀平 钟仙龙 项丽 徐安利 |
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| 作者单位： | 丽水学院化学与生命科学学院,浙江,丽水,323000 |
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| 基金项目： | 浙江省新苗人才计划项目(2010R429021); 浙江省A类重点学科项目(浙教高科[2009]165号) |
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| 摘 要： | [目的]测定乐清市铁皮石斛总黄酮的含量。[方法]以芦丁为对照品，采用NaNO2-A1（NO3）3-NaOH显色，在510nm波长处用紫外分光光度法测定总黄酮的含量。[结果]芦丁在O．0111～0．0667mg／ml浓度范围内与吸光度呈良好线性关系（R2=0．9992）；样品溶液在12h之内稳定性良好，RSD值为0．15％；建立的方法重复性良好，其RSD值为3．12％。[结论]该方法简便、准确、稳定，可用于铁皮石斛中总黄酮含量的测定。
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| 关 键 词： | 铁皮石斛（Dendrobium officinale Kimura et Migo） 比色法 总黄酮 |
Determination of the Content of Total Flavonoids in Dendrobium officinale Kimura et Migo from Yueqing |
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| WU Jia-wen et al. Determination of the Content of Total Flavonoids in Dendrobium officinale Kimura et Migo from Yueqing[J]. Journal of Anhui Agricultural Sciences, 2011, 39(18): 10856+10884-10856,10884 |
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| Authors: | WU Jia-wen et al |
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| Affiliation: | WU Jia-wen et al(College of Chemistry and Life Sciences,Lishui University,Lishui,Zhejiang 323000) |
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| Abstract: | [Objective] To determine the content of total flavonoids in Dendrobium officinale Kimura et Migo from Yueqing.[Method] Using rutin as reference substance,contents of total flavonoids in Dendrobium officinale Kimura et Migo from Yueqing were measured at 510 nm by UV spectrophotometry colored with NaNO2-Al(NO3)3-NaOH.[Results] Rutin concentration was linear with absorbance in the range of 0.011 1 to 0.066 7 mg/ml(R2=0.999 2);sample solution was stable within 12 h with RSD value of 0.15%;the established method... |
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| Keywords: | Dendrobium officinale Kimura et Migo Colorimetric method Total flavonoids |
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