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乐清市铁皮石斛总黄酮含量的测定
引用本文:吴佳雯,鲍佳路,吕耀平,钟仙龙,项丽,徐安利. 乐清市铁皮石斛总黄酮含量的测定[J]. 安徽农业科学, 2011, 39(18): 10856+10884-10856,10884
作者姓名:吴佳雯  鲍佳路  吕耀平  钟仙龙  项丽  徐安利
作者单位:丽水学院化学与生命科学学院,浙江,丽水,323000
基金项目:浙江省新苗人才计划项目(2010R429021); 浙江省A类重点学科项目(浙教高科[2009]165号)
摘    要:[目的]测定乐清市铁皮石斛总黄酮的含量。[方法]以芦丁为对照品,采用NaNO2-A1(NO3)3-NaOH显色,在510nm波长处用紫外分光光度法测定总黄酮的含量。[结果]芦丁在O.0111~0.0667mg/ml浓度范围内与吸光度呈良好线性关系(R2=0.9992);样品溶液在12h之内稳定性良好,RSD值为0.15%;建立的方法重复性良好,其RSD值为3.12%。[结论]该方法简便、准确、稳定,可用于铁皮石斛中总黄酮含量的测定。

关 键 词:铁皮石斛(Dendrobium  officinale  Kimura  et  Migo)  比色法  总黄酮

Determination of the Content of Total Flavonoids in Dendrobium officinale Kimura et Migo from Yueqing
WU Jia-wen et al. Determination of the Content of Total Flavonoids in Dendrobium officinale Kimura et Migo from Yueqing[J]. Journal of Anhui Agricultural Sciences, 2011, 39(18): 10856+10884-10856,10884
Authors:WU Jia-wen et al
Affiliation:WU Jia-wen et al(College of Chemistry and Life Sciences,Lishui University,Lishui,Zhejiang 323000)
Abstract:[Objective] To determine the content of total flavonoids in Dendrobium officinale Kimura et Migo from Yueqing.[Method] Using rutin as reference substance,contents of total flavonoids in Dendrobium officinale Kimura et Migo from Yueqing were measured at 510 nm by UV spectrophotometry colored with NaNO2-Al(NO3)3-NaOH.[Results] Rutin concentration was linear with absorbance in the range of 0.011 1 to 0.066 7 mg/ml(R2=0.999 2);sample solution was stable within 12 h with RSD value of 0.15%;the established method...
Keywords:Dendrobium officinale Kimura et Migo  Colorimetric method  Total flavonoids  
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