透明质酸发酵过程中底物抑制及解除抑制的方法研究

目的]探求获取高产量透明质酸的发酵方法。方法]在微生物发酵法生产透明质酸的过程中，探索其底物浓度对发酵的影响。结果]葡萄糖对透明质酸发酵的影响最大，低浓度的葡萄糖只能合成少量菌体和透明质酸，高浓度的葡萄糖会抑制茵体的生长和透明质酸的形成，探索试验发现，分批补料发酵可以解除底物抑制，其具体方法为在发酵开始前加入2％的葡萄糖，14h时加2％的葡萄糖，22h时再加2％的葡萄糖。结论]在微生物发酵过程中，分批补料发酵能够解除底物抑制，得到相对分子质量较大的透明质酸，且产量高。

Study on the Substrate Inhibition and the Disarming Inhibition Method in the Fermentation Process of Hyaluronic Acid

Objective] To search and obtain the fermentation method of hyaluronic acid with the high yield. Method] In the production process of hyaluronic acid by the microbial fermentation method, the influence of substrate concentration on the fermentation was explored. Result] The glucose had the biggest influence on the hyaluronic acid fermentation. The low concentration of glucose only compound a few bacteria and hyaluronic acid, and the high concentration of glucose could inhibit the growth of bacteria and th...