| 普通荞麦染色体的原位PCR技术研究 |
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| 引用本文： | 李分龙,陈庆富. 普通荞麦染色体的原位PCR技术研究[J]. 安徽农业科学, 2011, 0(17): 10135-10138 |
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| 作者姓名： | 李分龙 陈庆富 |
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| 作者单位： | 贵州师范大学生命科学学院植物遗传育种研究所; |
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| 基金项目： | 国家燕麦荞麦现代农业产业体系荞麦育种岗位科学资金项目(CARS-08-A4); 国家自然科学基金项目(31060207,30471116); 贵州省农业攻关项目(黔科合NY字[2010]3094); 贵州省动植物育种专项(黔农育专字[2010]023号) |
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| 摘 要： | [目的]探索一种适合荞麦的简单易行的染色体原位PCR技术。[方法]采用16S套式引物、4．5S套式引物与psbA引物，以栽培甜荞为材料，进行染色体原位PCR、原位套式PCR与多次原位PCR试验。[结果]高温干燥可以起到与包埋类似的作用；染色体的原位套式PCR效果比原位PCR明显，多次原位PCR次数为5-6效果较佳。16S引物和4．5S引物均显示了4对信号，但位置不同；而psbA引物是单拷贝的，仅显示出1对信号。根据这些信号的位置差异可以区分普通荞麦的5对染色体。[结论]所使用的荞麦染色体原位PCR技术简单易行。
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| 关 键 词： | 栽培甜荞 IS—PCR 16S rDNA 4．5S rDNA psbA． |
The In Situ PCR Technology on Chromosome of Common Buckwheat |
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| LI Fen-long et al. The In Situ PCR Technology on Chromosome of Common Buckwheat[J]. Journal of Anhui Agricultural Sciences, 2011, 0(17): 10135-10138 |
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| Authors: | LI Fen-long et al |
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| Affiliation: | LI Fen-long et al (Institute of Plant Genetics and Breeding,School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550001) |
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| Abstract: | [Objective] The paper aimed to explore a simple in-situ PCR technology for buckwheat. [Method] Common buckwheat was taken as experimental material. By using 16S and 4.5S nested primers and psbA primer, the in-situ PCR, nested in-situ PCR and multiple in-situ PCR were carried out respectively. [Result] High-temperature drying treatment had the effects similar to that of embedding methods. The effect of the nested in-situ PCR is better than that as usual. A better result could be obtained when the multiple in... |
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| Keywords: | Fagopyrum esculentum IS-PCR 16S rDNA 4.5S rDNA psbA. |
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