| 应用抑制差减杂交技术分离水稻早衰差异表达基因的初步研究 |
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| 引用本文： | 蒲志刚,王平,张志勇,蔡平钟,阎文昭,向跃武,张志雄. 应用抑制差减杂交技术分离水稻早衰差异表达基因的初步研究[J]. 西南农业学报, 2012, 25(2): 360-364 |
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| 作者姓名： | 蒲志刚 王平 张志勇 蔡平钟 阎文昭 向跃武 张志雄 |
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| 作者单位： | 四川省农业科学院生物技术核技术研究所,四川成都,610066 |
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| 基金项目： | 四川省农业科学院青年优秀论文基金 |
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| 摘 要： | 通过Co60辐射诱变获得了水稻早衰突变体材料H04002-9,以突变体为测试方,野生型为驱动方在早衰性状出现时构建了水稻早衰差异表达基因文库,差异片段克隆、测序后通过GeneBank数据库分析表明,突变体编码腺苷甲硫氨酸脱羧酶的Os02g0611200基因中有4个碱基发生了变异,引起了翻译出的蛋白质序列中3个氨基酸发生改变,使腺苷甲硫氨酸脱羧酶的活性发生了变化,因此初步推测Os02g0611200基因的表达量与水稻早衰具有相关性。
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| 关 键 词： | 水稻 早衰 差异显示 文库 |
Isolation of Specifically Expressed Genes in Rice Presenility by Using Suppression Subtraction Hybridization |
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| PU Zhi-gang , WANG Ping , ZHANG Zhi-yong , CAI Ping-zhong , YAN Wen-zhao , XIANG Yue-wu , ZHANG Zhi-xiong. Isolation of Specifically Expressed Genes in Rice Presenility by Using Suppression Subtraction Hybridization[J]. Southwest China Journal of Agricultural Sciences, 2012, 25(2): 360-364 |
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| Authors: | PU Zhi-gang WANG Ping ZHANG Zhi-yong CAI Ping-zhong YAN Wen-zhao XIANG Yue-wu ZHANG Zhi-xiong |
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| Affiliation: | (Institute of Biotechnology and Nuclear,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu,610066,China) |
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| Abstract: | Premature senescence mutant H04002-9 obtained by Co-γ radiation-induced mutation was used as the material to construct the rice premature senility differentially expressed genes library when premature senility characters occurred.Differential fragment was clonged and sequenced,then was analyzed by GeneBank database.The results showed that 4 bases in Os02g0611200 that coded adenosylmethionine decarboxylase had mutations that led to that 3 amino acids changed.The acativity of adenosylmethionine decarboxylase also was changed.The preliminary conclusion was drawn that the expression of Os02g0611200 gene had correlation with the rice premature senility |
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| Keywords: | Rice Presenility suppression subtractive cDNA library Gene |
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