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| 渤海黑牛BOLA-DQA2基因SNPs多态性与生长性状的关联性分析 | |
| 引用本文： | 孙菲菲,刘桂芬,万发春,宋恩亮,刘晓牧,谭秀文,孙国强. 渤海黑牛BOLA-DQA2基因SNPs多态性与生长性状的关联性分析[J]. 西南农业学报, 2012, 25(1): 271-275 |
| 作者姓名： | 孙菲菲 刘桂芬 万发春 宋恩亮 刘晓牧 谭秀文 孙国强 |
| 作者单位： | 1. 青岛农业大学动物科技学院,山东青岛266109;山东省畜禽疫病防治与繁育重点实验室,山东济南250100 2. 山东省农业科学院畜牧兽医研究所,山东济南250100;山东省畜禽疫病防治与繁育重点实验室,山东济南250100 3. 青岛农业大学动物科技学院,山东青岛,266109 |
| 基金项目： | 山东黑牛快速扩繁技术及其推广利用(省农业科技成果转化项目);山东省农业良种工程2010LZ012“优质肉牛新品种培育”;现代农业(肉牛)产业体系;肉牛优异品质种质创新利用研究;山东省中青年奖励基金(BS2010SW026) |
| 摘 要： | 本试验采用PCR-SSCP方法对渤海黑牛BOLA-DQA2基因的编码区进行分析,研究渤海黑牛BOLA-DQA2基因多态性与生产性状的关系。研究表明,外显子1和外显子5处没有发现多态性位点,外显子2处发现两个多态位点产生4种基因型,外显子3处发现1个多态位点产生3种基因型,外显子4处发现1个多态位点产生3种基因型。外显子2中基因型AA所对应的个体头长、额宽、体高、十字部高显著高于基因型BB、AB、BC(P<0.05),其它生长性状均无显著差异。外显子3、外显子4中生产性状在AA、BB、AB这3种基因型上均无显著差异。DQA2外显子2中等位基因A对渤海黑牛的生产性状起关键作用,可以为渤海黑牛品种选育提供一定的参考。 |
| 关 键 词： | BOLA-DQA2 PCR-SSCP 生长性状 渤海黑牛 |
Association of PCR-SSCP Polymorphisms of BOLA-DQA2 Gene with Growth Traits of Chinese Bohai Black Cattle |
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| SUN Fei-fei , LIU Gui-fen , WAN Fa-chun , SONG En-liang , LIU Xiao-mu , TAN Xiu-wen , SUN Guo-qiang. Association of PCR-SSCP Polymorphisms of BOLA-DQA2 Gene with Growth Traits of Chinese Bohai Black Cattle[J]. Southwest China Journal of Agricultural Sciences, 2012, 25(1): 271-275 | |
| Authors: | SUN Fei-fei LIU Gui-fen WAN Fa-chun SONG En-liang LIU Xiao-mu TAN Xiu-wen SUN Guo-qiang |
| Affiliation: | 1 (1.Qingdao Agricultural University,College of Animal Science and Technology,Shandong Qingdao 266109, China;2.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences, Shandong Jinan 250100,China;3.Shandong Key Laboratory of Animal Disease Control and Breeding,Shandong Jinan 250100,China) |
| Abstract: | The polymorphism of code region in BOLA-DQA2gene were analyzed by PCR-SSCP in Chinese Bohai Black Cattle.The results showed that two polymorphism sites were found in exon2 of DQA2,one polymorphism site was found in exon3 and exon4,but there were no polymorphism site in exon1 and exon5.There were four genotypes in exon2,there were three genotypes in exon3 and exon4.Head length,Frontal width,Body height and Height at the hip cross were higher for genotype AA than that for genotype AB,BC and BB,the other growth traits had no dominant difference in four genotypes.Growth traits had no dominant difference in three genotypes in exon3 and exon4.The mutation of exon2 of DQA2 in Chinese Bohai Black Cattle played an important role in growth traits,it could provide a basis for breed of selection Chinese Bohai Black Cattle. |
| Keywords: | BOLA-DQA2 PCR-SSCP Growth traits Bohai Black Cattle |
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