牛布鲁氏菌膜蛋白的免疫抗原筛选

试验旨在通过免疫蛋白质组学筛选布鲁氏菌保护性抗原。利用双向电泳技术对试验条件下培养的布鲁氏菌强毒株544A膜蛋白进行分离,结合Western blotting技术分别用豚鼠、牛抗布鲁氏菌血清寻找发生免疫反应的蛋白质。16个免疫蛋白点经胶内酶切、质谱(LC-MS/MS)进行鉴定。利用生物信息学工具发现这些蛋白不全是膜蛋白,还存在胞质蛋白,其功能涉及生物合成和物质代谢等领域。成功建立了布鲁氏菌膜蛋白的免疫蛋白质组学研究方法,为寻找保护性抗原及为新型疫苗抗原候选提供新思路。

Screening of Membrane Proteins of Brucella abortus

Application of immunoproteomic methods in research on Brucella abortus.Two-dimension electrophoresis was used to separate the membrane protein of Brucella abortus strain 544A grown under laboratory condition,and Western blotting was used to screen immunogenic proteins by serum of guinea and bov anti-Brucella abortus IgG.After ingel digestion,12 immunoreactive spots were identified by LC MS/MS.All the proteins were searched by the bioimformation tools,there were not only membrane proteins,but also cytoplasmic proteins.Most of them were involved in protein synthesis and metabolism.The immunoproteomic methods can be successfully applied in identifying immunogenic proteins of Brucella,which helps to search for other immunogenic proteins and lay a foundation for searching vaccine candidate antigens in future.