| 产脂肪酶菌株的筛选及其脂肪酶的双水相分离 |
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| 引用本文： | 陈利梅,李德茂. 产脂肪酶菌株的筛选及其脂肪酶的双水相分离[J]. 湖北农业科学, 2010, 49(2) |
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| 作者姓名： | 陈利梅 李德茂 |
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| 作者单位： | 聊城大学农学院食品科学与工程系,山东,聊城,252059 |
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| 摘 要： | 从土壤中筛选到了两株产脂肪酶的菌株真菌A和真菌B。测定二者酶活,并对真菌B所产脂肪酶的双水相萃取体系进行了优化。结果表明,真菌A酶活为26.5U/mL,真菌B酶活为15.0U/mL,而真菌A比酶活为6618.62U/g,真菌B比酶活为7332.20U/g,选取真菌B为研究对象。最佳PEG分子量为2000,最佳PEG浓度为200g/L,最佳硫酸铵浓度为250g/L。
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| 关 键 词： | 脂肪酶 发酵 酶活 双水相 |
Screening of Lipase-producing Microorganism and Lipase Extraction by Two Aqueous Two-phase Systems |
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| CHEN Li-mei,LI De-mao. Screening of Lipase-producing Microorganism and Lipase Extraction by Two Aqueous Two-phase Systems[J]. Hubei Agricultural Sciences, 2010, 49(2) |
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| Authors: | CHEN Li-mei LI De-mao |
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| Abstract: | Two lipase-producing strains,named fungus A and B,were isolated from soil samples.The enzyme activities and specific activities of fungus A and B were determined.The results indicated that the lipase activity of A was higher than that of B (The lipase activity of A was 26.5U and B was 15.0U).And the specific activity of B was higher than that of A (The specific activity of A was 6 618.62 U/g and B was 7 332.20 U/g).As a result,fungus B was selected to optimize the aqueous two-phase system.The optimal conditions of extraction showed as follows:the molecular weight of PEG was 2 000,the concentration of PEG was 25%,and the concentration of (NH_4)_2SO_4 was 20%. |
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| Keywords: | lipase fermentation enzyme activity aqueous two-phase |
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