Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool |
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Authors: | McBeath S J Ellis L M Cook P F Wilson L Urquhart K L Bricknell I R |
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Institution: | FRS Marine Laboratory, Aberdeen, UK. |
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Abstract: | Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase-based immunoassay, and Western blot. |
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Keywords: | diagnostic tests enzyme immunoassay indirect fluorescent antibody test infectious salmon anaemia virus monoclonal antibody polyclonal antisera |
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