| 长春花碱在牛体外受精胚中染色体标本处理及性别分析 |
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| 引用本文： | 朱淑文,吉泽绿,李培昌.长春花碱在牛体外受精胚中染色体标本处理及性别分析[J].西南农业大学学报,2001,23(4):333-335. |
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| 作者姓名： | 朱淑文 吉泽绿 李培昌 |
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| 作者单位： | 朱淑文（上海交通大学农学院动物科学系上海201101）
吉泽绿（日本国字都宫大学农学部畜产学科日本321-8505）
李培昌（日本国字都宫大学农学部畜产学科日本321-8505） |
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| 摘 要： | 长春花碱是一种常用的细胞分裂阻止剂,在细胞遗传学分析及体外受精胚的性别鉴定等方面有着重要意义.该研究对牛体外受精胚的第一卵裂期染色体标本制作及性比进行了分析.废弃牛卵巢内卵子经体外成熟培养,体外受精20h后,在培养基中添加100ng/ml长春花碱处理8h或10h.结果表明,用长春花碱处理8h的胚胎标本大部分含有可分析的中期分裂像并可进行性别判断,明显高于10h处理的胚胎标本(P<0.05).性比分析表明,牛体外受精的胚胎的雄性发生率略高于雌性发生率.
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| 关 键 词： | 牛卵子 染色体 长春花碱 牛 体外受精胚 性别 |
| 文章编号： | 1000-2642(2001)04-0333-03 |
| 修稿时间： | 2001年2月14日 |
SEXING AND VINBLASSTINE TREATMENT FOR CHROMOSOME PREPARATION IN BOVINE EMBRYOS DERIVED FROM IN VITRO FERTILIZATION |
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| ZHU Shu-wen ,Yoshizawa Mdor ,LI Peo-chang.SEXING AND VINBLASSTINE TREATMENT FOR CHROMOSOME PREPARATION IN BOVINE EMBRYOS DERIVED FROM IN VITRO FERTILIZATION[J].Journal of Southwest Agricultural University,2001,23(4):333-335. |
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| Authors: | ZHU Shu-wen Yoshizawa Mdor LI Peo-chang |
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| Institution: | ZHU Shu-wen 1,Yoshizawa Mdor 2,LI Peo-chang 2 |
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| Abstract: | The required concentration and time duration of vinblastine sulfate treatment were determined for successful cytogenetic diagnosis, such as normality and sex in bovine embryos derived from in vitro fertilization. Twenty hours after insemination, the embryos were placed on a medium containing 100 ng/ml vinblastine sulfate and cultured for 8 and 10 hours. The rate of the embryos with mitotic metaphase figures and sex ratio in the medium cultured for 8 hours was significantly higher than that cultured for 10 hours ( P <0.05). A sex ratio with a slight male advantage was found in the present study. |
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| Keywords: | bovine oocyte first cleavage chromosome sex ratio |
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