| Molecular Characterization of a Triticum durum-Haynaldia villosa Amphiploid and Its Derivatives for Resistance to Gaeumannomyces graminis var. tritici |
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| 作者姓名： | HUANGDa-hui LIN Zhi-shan CHEN Xiao ZHANG Zeng-yan CHEN Cai-ceng CHENG Shun-he XIN Zhi-yong |
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| 作者单位： | [1]Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture~Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China [2]Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, P.R. China [3]Institute of Agricultural Science of Lixiahe District, Yangzhou 225007, P.R.China |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家高技术研究发展计划(863计划) |
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| 摘 要： | Take-all is a serious disease found in wheat across the world. Haynaldia villosa is considered to be resistant to take-all at a high level. TH3 was an amphiploid （2n =42, AABBVV） between Triticum durum and Haynaldia viUosa with significant resistance to take-all fungus isolated from China. In greenhouse experiment, the derivatives of the hybrid between wheat and TH3 showed better resistance to take-all than that of the wheat control. One of the derivatives named HW918-5 was selected for further analysis. Cytological and genomic in situ hybridization （GISH） analysis indicated that a monotelosome originated from H. villosa existed in the genome of the offspring of the line HW918-5. The monotelosome with promising resistant gene for take-all was located on the 3V chromosome of H. villosa in the further PCR-based molecular analysis.
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| 关 键 词： | 小麦全蚀病 抗病性 簇毛麦 双倍体 分子特征 基因组原位杂交 |
| 收稿时间： | 2006-09-26 |
| 修稿时间： | 2006-12-27 |
Molecular Characterization of a Triticum durum-Haynaldia villosa Amphiploid and Its Derivatives for Resistance to Gaeumannomyces graminis var. tritici |
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| HUANGDa-hui LIN Zhi-shan CHEN Xiao ZHANG Zeng-yan CHEN Cai-ceng CHENG Shun-he XIN Zhi-yong.Molecular Characterization of a Triticum durum-Haynaldia villosa Amphiploid and Its Derivatives for Resistance to Gaeumannomyces graminis var. tritici[J].Agricultural Sciences in China,2007,6(5):513-521. |
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| Authors: | HUANG Da-hui LIN Zhi-shan CHEN Xiao ZHANG Zeng-yan CHEN Cai-ceng CHENG Shun-he XIN Zhi-yong |
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| Institution: | 1. Department of Botany, Sidho-Kanho-Birsha University, Ranchi Road, Purulia, 723104, India;2. Centre of Advanced Study, Department of Botany, University of Calcutta, 35, B.C. Road, Kolkata, 700019, India;3. School of Environment, Earth and Ecosystem Sciences, The Open University, Milton Keynes MK7 6AA, UK;4. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China;5. Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, China |
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| Abstract: | Take-all is a serious disease found in wheat across the world. Haynaldia villosa is considered to be resistant to take-all at a high level. TH3 was an amphiploid (2n=42, AABBVV) between Triticum durum and Haynaldia villosa with significant resistance to take-all fungus isolated from China. In greenhouse experiment, the derivatives of the hybrid between wheat and TH3 showed better resistance to take-all than that of the wheat control. One of the derivatives named HW918-5 was selected for further analysis. Cytological and genomic in situ hybridization (GISH) analysis indicated that a monotelosome originated from H. villosa existed in the genome of the offspring of the line HW918-5. The monotelosome with promising resistant gene for take-all was located on the 3V chromosome of H. villosa in the further PCR-based molecular analysis. |
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| Keywords: | AFLP Gaeumannomyces graminis var tritici genomic in situ hybridization Haynaldia villosa Triticum aestivum |
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