| 地黄枯萎病拮抗菌S15发酵条件的初步研究 |
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| 引用本文： | 张艳丽,高玉红,李洪连.地黄枯萎病拮抗菌S15发酵条件的初步研究[J].北方园艺,2012(17):127-129. |
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| 作者姓名： | 张艳丽 高玉红 李洪连 |
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| 作者单位： | 郑州职业技术学院生物工程系,河南郑州,450121;河南农业大学,河南郑州,450002 |
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| 摘 要： | 从地黄田土壤中筛选到1株对地黄枯萎病有较强拮抗作用的菌株S15,并对S15进行发酵条件的初步研究。结果表明:生防菌S15发酵最合适的条件为:28℃、pH 7.5的黄豆饼粉浸出液1%,葡萄糖10g,NaCl 2.5g,CaCO32g,蛋白胨3g,蒸馏水1 000mL的培养液中以170r/min的速度振荡培养4d。
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| 关 键 词： | 地黄枯萎病 发酵条件 发酵培养基 |
Primary Study on Fermentation Conditions of Antagonistic Strain S15 Against Rehmanniae Fusarium Wilt |
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| ZHANG Yan-li
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GAO Yu-hong
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LI Hong-lian.Primary Study on Fermentation Conditions of Antagonistic Strain S15 Against Rehmanniae Fusarium Wilt[J].Northern Horticulture,2012(17):127-129. |
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| Authors: | ZHANG Yan-li GAO Yu-hong LI Hong-lian |
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| Institution: | 1.Department of Biological Engineering,Zhengzhou Technical College,Zhengzhou,Henan 450121;2.Henan Agricultural University,Zhengzhou,Henan 450002) |
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| Abstract: | An actinomycete strain S15 antagonistic against Rehmanniae Fusarium Wilt was isolated from the soil in Rehmanniae field and the fermentation conditions were primary investigated.The results showed that the optimal fermentation conditions were lixivium of soybean cake 1%,glucose 10 g,NaCl 2.5 g,CaCO3 2 g,peptone 3 g,distilled water 1 000 mL,pH 7.5,temperature 28℃,170 r/min,fermenting 4 days. |
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| Keywords: | rehmanniae fusarium wilt fermentation condition fermentation medium |
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