| 不同光质对喜树愈伤组织形成及分化潜质影响 |
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| 引用本文： | 陈博,陈哲皓,王利琳,胡江琴.不同光质对喜树愈伤组织形成及分化潜质影响[J].北方园艺,2012(17):112-115. |
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| 作者姓名： | 陈博 陈哲皓 王利琳 胡江琴 |
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| 作者单位： | 杭州师范大学生命与环境科学学院,浙江杭州,310036 |
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| 基金项目： | 浙江省自然科学基金资助项目 |
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| 摘 要： | 以喜树无菌苗的茎、叶为外植体,研究了蓝、白、红3种光质下,MS、1/2MS、SH、WPM4种培养基对喜树愈伤组织形成及分化潜质影响。结果表明:蓝光下SH+1.0mg/L NAA+0.075mg/L TDZ培养基上愈伤组织诱导效果最好;愈伤组织继代后在蓝光下第7天时,SOD酶活最高,POD酶活最低,含水量最低,分化的潜力最大。
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| 关 键 词： | 光质 愈伤组织 分化潜力 喜树 |
Effects of Different Light Quality on the Formation and Regenerative Potentialities of Calli of Camptotheca acuminata |
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| CHEN Bo
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CHEN Zhe-hao
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WANG Li-lin
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HU Jiang-qin.Effects of Different Light Quality on the Formation and Regenerative Potentialities of Calli of Camptotheca acuminata[J].Northern Horticulture,2012(17):112-115. |
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| Authors: | CHEN Bo CHEN Zhe-hao WANG Li-lin HU Jiang-qin |
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| Institution: | (College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou,Zhejiang 310036) |
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| Abstract: | Taking the stems and leaves of Camptotheca acuminata as the explants,the formation and regenerative potentialities of calli from Camptotheca acuminata under different light quality(white/blue/red) and four media(SH,MS,WPM and 1/2MS) with 0.075 mg/L TDZ and 1.0 mg/L NAA were studied.The results showed that the calli cultured under blue light on SH medium had the best multiplication conditions.At the 7th day of subculture under blue light,the calli had the highest SOD activity and the lowest POD activity as well as water content,which might suggest a highly potential of regeneration of them.These results provided fundamental basis for the further research of genetic transformation,cell culture,plant regeneration and secondary metabolites acquisition in Camptotheca acuminata. |
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| Keywords: | light quality callus regenerative potentiality Camptotheca acuminata |
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