铁炮百合花挥发油不同提取方法的GC-MS研究

采用气液相连续萃取法和水蒸气蒸馏法对铁炮百合花的挥发油进行提取,气相色谱-质谱法分离并鉴定了其化学成分;并用峰面积归一化法测定了各成分的百分含量。结果表明:2种方法共分离出57个组分,确定了其中52种化合物;其主要成分为烯、醇、酯、醛、酸和烷类化合物。与水蒸气蒸馏法相比,气液相连续萃取法多萃取出14种化合物,增加的化合物中低沸点、相对分子质量小的成分较多。另外,2种方法共有的低沸点化合物中,气液相连续萃取法萃取出的化合物含量均较高,如α?萜品醇(0.40%、3.49%)、(E)-3,7-二甲基-2,6-辛二烯-1-醇(0.35%、2.60%)、3,7,11-三甲基-1,6,10-十二碳三烯-3-醇(10.40%、19.76%)等。

Study on Volatile Oils of Lilium longiflorum by Different Extraction Methods with GC-MS

The volatile oils was extracted by steam distillation method and gas liquid phase continual extraction method from Lilium longiflorum and by gas chromatography mass spectrometry(GC-MS) were analyzed.Finally the area normalization was used to measure the relative percentages of each of the components.The results showed that 57 components were separated and 52 components were identified.The main components were alkene,alcohol,ester,aldehyde,acid and alkane series.compared with steam distillation method,more than 14 components with lower boiling points and small relative molecular mass were extracted by gas liquid phase continual extraction method.The relative percentages were higher of lower boiling points components by gas liquid phase continual extraction method,for example:p-menth-1-en-8-ol(0.40%,3.49%),(E)-3,7-dimethyl-2,6-Octadien-1-ol(0.35%,2.60%),3,7,11-trimethyl-1,6,10-Dodecatrien-3-ol(10.40%,19.76%).