壳低聚糖浸种对镉胁迫下小白菜幼苗部分生理生化特性的影响

分别以0、10、50、100、150、200及300mg/L 7种浓度的壳低聚糖溶液对小白菜种子浸种24h,培养箱培养7d后,选长势一致幼苗移栽于1.5kg重的基质中,1kg基质含浓度为2.5mmol/L的Cd2+溶液20mL,15d后测定各项生理指标,研究壳低聚糖对镉胁迫下小白菜幼苗生长的影响。结果表明:经壳低聚糖浸种处理后,与对照相比,SOD、POD和CAT活性增大;根系活力、可溶性糖含量和叶绿素含量升高;MDA含量和叶片相对电导率降低。因此,适宜浓度壳低聚糖浸种处理可增强小白菜幼苗的抗重金属镉毒害性,且以150～200mg/L壳低聚糖浸种处理的缓解镉毒害效果最明显。

Influence of Oligochitosan on Some Physiological and Biochemical Characteristics of Chinese Cabbage under the Stress of Cadmium

The seed of Chinese cabbage were treated with 0,10,50,100,150,200 and 300 mg/L of oligochitosan for 24 h,exposed to matrix that include 20 mL 2.5 mmol/L Cd2+ solution of the 1.5 kg matrix potted plants for 7 days and then determined each index after 15 days the effects of oligochitosan on the stress of cadmium activity.The results showed that compared with the control,the activities of three protective enzymes,such as superoxide SOD,POD and CAT increased;root activity,the content of soluble sugar and chlorophyll content rised;while the MDA content and leaf relative electrical conductivity decreased in the leave of treated plants.The concentration of oligochitosan at 150 to 200 mg/L showed the most effective result.