黑果腺肋花楸组培苗增殖的初步研究

以黑果腺肋花楸带侧芽的茎段为外植体,研究了培养基种类、激素种类及浓度、糖源种类、外植体类型及光照强度对组培苗增殖的影响。结果表明:MS为适合黑果腺肋花楸组培苗生长的基本培养基;激素处理为BA 0.5mg/L,NAA 0.3mg/L时植株生长健壮,鲜重、干重分别达到最大值,增殖系数达到8.0;糖源为白糖时鲜重、干重分别达到926.6、182.1mg,植株生长健壮,增殖系数达8.4;外植体宜选取茎尖部(2.0±0.1)cm,增殖系数可达8.8;光照强度在1 600～2 600lx条件下有利于增殖及生长。

Primary Study on Tissue Culture Proliferation of Aronia melanocarpa

Taking stems with lateral buds of Aronia melanocarpa as explants,the effects of culture media,hormone types and concentrations,sugar types,explant types and illumination intensities on tissue culture proliferation were studied.The results showed that MS was the suitable basic medium.The hormone treatment of BA 0.5 mg/L,NAA 0.3 mg/L was better for growth and proliferation,resulting in fresh weight and dry weight reaching the maximum respectively with a proliferation index of 8.0.White sugar was considered as the suitable source for Aronia melanocarpa,with fresh weight 926.6 mg and dry weight 182.1 mg.The stem tip of(2.0±0.1)cm was the best part for explant,leading the proliferation up to 8.8 cm and the beneficial illumination intensity was 1 600~2 600 lx.