转基因植物标准物质研究进展

转基因产品的安全性一直都备受关注,因此转基因产品的定性、定量检测越来越重要,而转基因标准物质的使用是转基因产品检测结果有效和可比的重要保证.本文针对国内外转基因植物标准物质的研究现状及相关技术进行综述,重点介绍了转基因植物标准物质的种类、转基因标准物质定值技术,分析了转基因标准物质研制过程中的关键点.更重要的是综述并提出了质粒DNA分子标准物质的定值模式,包括如何合理评价质粒分子可替代性问题,此外还总结了目前国外转基因标准物质的种类,目的是为我国转基因植物标准物质研制和相关研究提供有价值的参考.

Advance in Developing Genetically Modified Plant Reference Material

In the past two decades,many countries have been paying more attention to safety of genetically modified(GM) product,and thus,qualitative and quantitative test of GM products become more and more important.The test result can be more comparable and reliable by using GM reference material.This paper reviews the research progress of GM reference material and related techniques:it introduces the variety of GM reference material and techniques of value assignment for GM reference material,and also evaluates the key procedures in developing GM reference material.More importantly,it is concluded that how to assign the reference value for the plasmid reference material including the commutability between the plasmid and genomic DNA.Additionally,it summarizes the current international GM certified reference material.This aims to provide some valuable reference for developing GM reference material in China.