利用EST序列鉴定葡萄应答外源赤霉素的基因

为了利用NCBI上大量的葡萄(Vitis viniferaL.)EST序列进行葡萄应答外源赤霉素基因的信息挖掘,通过本地化Blast、Blast2go等工具以及其他生物信息学工具和数据库,对NCBI上经过赤霉素(gibberellins,GA)诱导后的葡萄EST序列进行处理,获得了45条仅在赤霉素处理后表达的无冗余EST序列。Blastx注释结果显示,45条序列中有25条序列注释为假定蛋白或未知蛋白(约占55.6%),14条序列无注释信息(约占31.1%),6条序列(13.1%)注释有推定功能,其中包括整合酶蛋白(EE077049)、SPX结构域基因1(EE085000)、丝氨酸羧肽酶类44蛋白(EE091188)、CHY1(EE092187)、假尿苷合酶/转运蛋白(EE106096)、钾离子通道蛋白(EE108944)。Blast2go分析显示,共有29条序列通过基因本体论(GO)注释180个GO号,分属于分子功能(molecula rfunction)、细胞成分(cellular component)、生物过程(biological process)三大类的不同水平。初步推断,在外源赤霉素对葡萄进行处理后,应答基因主要具有结合活性(46.34%)和催化活性(39.02%)两方面的分子功能。其作用空间分布为整个细胞(44.68%)或者细胞器(40.43%)中,并且主要通过参与细胞生理过程(28.57%)和代谢过程(25.71%)等一系列复杂的生理生化反应完成整个应答过程。为进一步研究赤霉素处理后导致葡萄的基因表达情况提供了基本资料。

Identification of Grape (Vitis vinifera L.) Genes from EST Sequences Responding to Exogenous Gibberellins Treatment

To identify grape(Vitis vinifera L.) genes in response to exogenous gibberellins(GA) treatment,a large number of grape EST sequences collected at NCBI were analyzed.Forty-five non-redundant EST sequences that only expressed after GA treatment were obtained by using local Blast,Blast2go and other programs.Blastx annotation results indicated that twenty-five sequences(55.6%) were of hypothetical or unknown protein,fourteen sequences(31.1%) did not have available information,and six sequences(13.1%) had predict functions.The six sequences with annotated functions carried genes encoding integrase(EE077049),SPX domain(EE085000),serine carboxypeptidase-like 44(EE091188),CHY1(EE092187),pseudouridine synthase/transporter(EE106096) and K+ channel protein(EE108944).Blast2go analysis showed that only twenty-nine sequences(64.4%) had available Gene Ontology(GO) annotations,belonging to categories of molecular function,cellular component or biological process.Taken together,the GA-responsive gene products mainly had binding activities(46.34%) or catalytic activities(39.02%),distributed in the whole cell(44.68%) or in specific organelles(40.43%),participated1 in cellular processes(28.57%) or metabolic processes(25.71%) in responding to the exogenous GA treatment.This study provides basic information for further analysis of gene expression in response to exogenous GA treatment.