| 纽荷尔脐橙CRTISO基因片段的克隆与分析 |
| |
| 引用本文： | 陶能国,张继红.纽荷尔脐橙CRTISO基因片段的克隆与分析[J].吉林农业大学学报,2008,30(1):28-30. |
| |
| 作者姓名： | 陶能国 张继红 |
| |
| 作者单位： | 湘潭大学化工学院,湘潭,411105 |
| |
| 基金项目： | 国家自然科学基金项目(30300241)，湘潭大学科研启动基金项目(06QDZ11)，湘潭大学跨学科交叉项目(06IND01) |
| |
| 摘 要： | 利用RT-PCR技术,从纽荷尔脐橙(Citrus sinensisOsbeck)果实cDNA中分离出类胡萝卜素异构酶(Carotenoid isomerase,CRTISO)基因片段。该片断长446 bp,编码148个氨基酸。用BLAST比较其氨基酸同源性发现,该片段氨基酸残基与伏令夏橙(C.sinensisOsbeck)、番茄(Lycopersicon esculentumMiller)和拟南芥(Ara-bidopsis thaliana)CRTISO的同源性分别为99%、85%和83%,与里斯本柠檬(C.limonBurm.F.)和温州蜜柑(C.unshiuMarc.)完全一致。半定量RT-PCR分析表明,CRTISO基因在8~11月份纽荷尔果实中均有表达,10月份的表达量最大。
|
| 关 键 词： | 纽荷尔脐橙 RT-PCR 类胡萝卜素异构酶 基因 |
| 文章编号： | 1000-5684(2008)01-0028-03 |
| 修稿时间： | 2007年3月29日 |
Cloning and Analysis of CRTISO Gene Fragment from Newhall Navel Orange |
| |
| TAO Neng-guo,ZHANG Ji-hong.Cloning and Analysis of CRTISO Gene Fragment from Newhall Navel Orange[J].Journal of Jilin Agricultural University,2008,30(1):28-30. |
| |
| Authors: | TAO Neng-guo ZHANG Ji-hong |
| |
| Abstract: | The cDNA sequence for carotenoid isomerase(CRTISO) has been cloned from fruits of Newhall navel orange by RT-PCR technique.Sequencing results revealed that it shared the length for 446bp,encoding 148 amino acids.BLAST results showed that the deduced amino acid shared 100% identities with those of Lisbon limon and Satsuma Mandarin,99% identities with Valencia sweet orange CRTISO,85% and 83 % identities with those of Lycopersicon esculentum Miller and Arabidopsis thaliana,respectively.Semi-quantitative RT-PCR results showed that expressions of CRTISO gene in fruits of Newhall navel orange were detected from August to November,with the highest one in October. |
| |
| Keywords: | Newhall navel orange RT-PCR carotenoid isomerase gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
