不同无机磷添加水平对混合瘤胃微生物体外发酵的影响

采用体外法研究无机磷添加浓度对瘤胃发酵的影响。培养液中设0mg/L、30mg/L、60mg/L、90mg/L、120mg/L、150mg/L、200mg/L、250mg/L、300mg/L、350mg/L共10个无机磷水平。体外发酵48h后终止培养,测定培养液的产气量、pH值、挥发性脂肪酸、干物质降解率、NDF降解率。结果表明:无机磷添加水平对48h总产气量和DM降解率影响显著(P0.05),随着无机磷添加水平的提高,48h总产气量和DM降解率显著提高,添加水平为200mg/L时达到最大值,分别为44.30mL和54.80%,随后下降。瘤胃培养液pH值随无机磷添加水平的增加而显著降低(P0.05),无机磷添加水平为200mg/L时达到最小值6.35,随后又上升。不同无机磷添加水平对乙酸、丙酸、丁酸和总VFA产量影响显著(P0.05),无机磷添加水平为200mg/L时,乙酸、丙酸、丁酸及总VFA产量最高,随后下降。

Effects of different inorganic phosphorus on in vitro fermentation of mixed ruminal microorganisms

The effects of rumen fermentation by adding inorganic P in vitro were studied, concentration of inorganic phosphorus were 0mg/L, 30mg/L, 60mg/L, 90mg/L, 120mg/L, 150mg/L, 200mg/L, 250mg/L, 300mg/L and 350mg/L. The gas production, pH, VFA, dry matter and NDF degradation of 48h fermentation in vitro were measured. The results as follow: different inorganic P levels significantly influenced the gas production and DM digestibility by 48h fermentation in vitro (P<0.05). The gas production and DM digestibility in...