暗纹东方鲀Amhr2基因外显子9的SNP7271检测及其与性别相关分析 |
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引用本文: | 金武,闻海波,杜兴伟,马学艳,顾若波,徐跑. 暗纹东方鲀Amhr2基因外显子9的SNP7271检测及其与性别相关分析[J]. 淡水渔业, 2016, 0(2): 99-102. DOI: 10.3969/j.issn.1000-6907.2016.02.017 |
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作者姓名: | 金武 闻海波 杜兴伟 马学艳 顾若波 徐跑 |
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作者单位: | 1. 农业部淡水渔业与种质资源利用重点实验室中国水产科学研究院淡水渔业研究中心,江苏无锡 214081; 南京农业大学渔业学院,江苏无锡 214182;2. 农业部淡水渔业与种质资源利用重点实验室中国水产科学研究院淡水渔业研究中心,江苏无锡,214081;3. 南京农业大学渔业学院,江苏无锡,214182 |
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基金项目: | 中国水产科学研究院淡水渔业研究中心中央级公益性科研院所基本科研业务费专项资金项目(2013JBFR07);江苏省水产三新工程项目(D2014-9) |
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摘 要: | 利用传统PCR和ABI 3730测序技术对暗纹东方鲀(Takifugu obscurus)Amhr2基因外显子9进行了SNP7271检测和测序分析,并对该SNP位点与暗纹东方鲀性别相关性进行了研究。结果显示,在SNP7271位点发生了G/C碱基突变,产生了CG、CC两种基因型。SNP7271和暗纹东方鲀性别存在显著相关性(P0.01)。基因型为CG的个体生理性别为雄性,基因型CC的个体生理性别为雌性。
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关 键 词: | Amhr2基因 暗纹东方鲀(Takifugu obscurus) 性别决定 基因型 |
SNP7271 detection of exton 9 of Amhr2 gene and its associations with sex association in the Takifugu obscurus |
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Abstract: | In order to identify gender of Takifugu obscurus before reproduction season and save the input of aquaculture of T.obscurus, SNP7271 of exton 9 of T.obscus Amhr2 gene were detected by PCR and DNA sequencing in present study . The correlation analysis between the polymorphism of Amhr2 and sex determination was studied .The results indicated that a G/C mutation at the position SNP7271 of the Amhr2 gene by sequencing and resulted in 2 genotype (GG, GC).There was significant correlation between SNP 7271 and the gender of T.obscurus.The genotype CG was male and the genotype CC was female. |
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Keywords: | Amhr2 gene Takifugu obscurus sex determination genotype |
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