| 斑茅SAMDC基因的克隆及其原核表达分析 |
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| 引用本文： | 蔡秋华,张积森,刘文荣,饶进,翁笑艳,陈如凯,张木清.斑茅SAMDC基因的克隆及其原核表达分析[J].福建稻麦科技,2009,27(1). |
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| 作者姓名： | 蔡秋华 张积森 刘文荣 饶进 翁笑艳 陈如凯 张木清 |
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| 作者单位： | 1. 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州,350002;福建省农业科学院水稻研究所,农业部闽台农作物种质资源利用重点开放实验室,福建福州,350019
2. 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州,350002 |
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| 摘 要： | 以斑茅(Erianthus arundinaceus)samdc基因的cDNA为基础,采用基因重组技术,将该基因按正确的阅读框架定向克隆于原核表达载体pET-29a(+)中,转化大肠杆菌BL21(DE3),用IPTG诱导表达,并对表达产物进行SDS-PAGE分析.结果表明:重组斑茅samdc基因在大肠杆菌中获得高效表达,其分子量约为43.281kDa.斑茅samdc基因原核表达载体的成功构建和重组斑茅SAMDC蛋白在大肠杆菌中的高效表达,为进一步研究其生物学功能奠定了基础.
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| 关 键 词： | 斑茅 表达载体构建 基因表达 |
Construction of Prokaryotic Expression Vector of Erianthus Arundinaceus Samdc and Its Expression in Escherichia Coil |
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| CAI Qiu-Hua,ZHANG Ji-Sen,LIU Wen-Rong,RAO Jin,WENG Xiao-Yan,CHEN Ru-Kai,ZHANG Mu-Qing.Construction of Prokaryotic Expression Vector of Erianthus Arundinaceus Samdc and Its Expression in Escherichia Coil[J].Fujian Science and Technology of Rice and Wheat,2009,27(1). |
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| Authors: | CAI Qiu-Hua ZHANG Ji-Sen LIU Wen-Rong RAO Jin WENG Xiao-Yan CHEN Ru-Kai ZHANG Mu-Qing |
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| Institution: | 1. Key Lab of Eco-Physiology and Genetic Improvement of Sugarcane under Ministry of Agriculture;Fujian Agri. & Forestry Univ. Fuzhou;350002;2.Rice Research Institute;Fujian Academy of Agricultural Sciences;Key laboratory of Crop Germplasm Utilization between Fujian and Taiwan;Ministry of Agriculture;P.R.China;Fuzhou 350019 |
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| Abstract: | Prokaryotic expression vector pET-SAMDC of Erianthus arundinaceus samdc gene cDNA was constructed by means of recombinant gene technology and expressed in Escherichia coil BL21 under induction of IPTG, the e xpressed products were detected by SDS-PAGE analysis. Result show: Recombinant samdc was efficiently expressed in E.coli BL21,it molecular weight was about 43.281Kda. The successful construction of prokaryotic expression vector c ontaining Erianthus arundinaceus samdc gene and effective expression of re... |
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| Keywords: | samdc |
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