正交试验优化红豆杉硒多糖的提取

以恩施高硒地区的珍贵药用植物红豆杉为研究对象,以料液比、水浴浸提时间以及微波处理时间为试验因素设计正交试验,优化了红豆杉硒多糖的提取分离纯化工艺.通过硫酸苯酚法测定红豆杉中硒多糖的含量,采用原子荧光法测定硒多糖中硒的含量,并以SPSS统计软件对正交试验结果进行处理和分析.结果表明,3因素对硒多糖提取率影响大小为料液比＞微波处理时间＞水提时间,且红豆杉中硒多糖的最佳提取条件为料液比1∶80(W/V)、水浴浸提时间30 min、微波处理时间5 min.在此条件下,测得红豆杉中硒多糖含量为1.96％,硒多糖中硒含量为0.0555 μg/g.

Extraction of Se-polysaccharides from Taxuschinensis by orthogonal test

In this thesis, Taxus chinensis, a kind of precious medicinal plant that grows in Enshi where it is a highselenium areas in China, was used as experimental material. Solid -liquid ratio, water extraction time and microwaveprocessing time were the three experimental factors, then orthogonal experiment was designed to find a more efficient wayto extract and separate Se-polysaccharide from T. chinensis. Based on that, the phenol sulfuric acid method was used todetect the content of Se-polysaccharide in T. chinensis, and the atomic fluorescence method was used to detect the contentof Se in Se -polysaccharide. In addition, statistical software, SPSS, was used to analyze and process the orthogonalexperiment results. The results showed that the best conditions to extract this kind of polysaccharide were as follows: thesolid-liquid ratio was 180 (W/V), water extraction time was 130 min, microwave processing time was 5 min. Among thesethree factors, the influence order on polysaccharide extraction rate was solid-liquid ratio>microwave processing time>waterextraction time. Under these conditions, the content of Se-polysaccharide was up to 1.96%, selenium content in Sepolysaccharidewas 0.0555 g/g.