| 家兔UCP1 第3外显子基因多态性研究 |
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| 引用本文： | 李维,谢海强,刘若余,林家栋,李思,杜雪琴,肖超能. 家兔UCP1 第3外显子基因多态性研究[J]. 广东农业科学, 2014, 41(24): 138-141 |
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| 作者姓名： | 李维 谢海强 刘若余 林家栋 李思 杜雪琴 肖超能 |
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| 作者单位： | 1. 贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室/贵州大学动物科学学院/贵州省动物遗传育种与繁殖重点实验室,贵州贵阳,550025
2. 贵阳市小河第三实验学校,贵州贵阳,550006 |
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| 基金项目： | 贵州省科技支撑计划项目 |
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| 摘 要： | 为了解兔肉质性状特征,给兔的品种选种选育提供理论依据,选择UCP1基因设计1对引物扩增其第3外显子及第2内含子部分序列,采用DNA池PCR反应,直接测序法快速检测比利时兔、新西兰兔和加利福尼亚兔3个兔品种UCP基因多态性.结果在新西兰兔中发现1个多态性位点(T387C),多态位点对UCP1基因的RNA结构产生影响,其最小自由能由-917.51 kJ/mol变为-927.12 kJ/mol,而其他2个品种没有检测到该突变.
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| 关 键 词： | UCP1 比利时兔 新西兰兔 加利福尼亚兔 多态性 |
Study on the SNP of UCP1 Exon3 in rabbits |
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| LI Wei,XIE Hai-qiang,LIU Ruo-yu,LIN Jia-dong,LI Si,DU Xue-qin,XIAO Chao-neng. Study on the SNP of UCP1 Exon3 in rabbits[J]. Guangdong Agricultural Sciences, 2014, 41(24): 138-141 |
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| Authors: | LI Wei XIE Hai-qiang LIU Ruo-yu LIN Jia-dong LI Si DU Xue-qin XIAO Chao-neng |
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| Abstract: | In order to deeply understand the meat quality and provide basic information and scientific proof for betterheredity selection of rabbit, the complete sequences of exon3 and part of intron2 region in UCP1 of rabbit were obtained bydesigning one pair of specific primers. DNA pools PCR and the direct sequencing were used to test the SNP site in Belgianrabbit, New Zealand rabbit and California rabbit. The results showed that only one SNP (G387C) site was found in NewZealand rabbit, and the polymorphism led to the changes of RNA secondary structure, its minimum free energy changedfrom -917.51 kJ/mol to -927.12 kJ/mol. However, there was no SNP site in the other two breeds. |
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| Keywords: | UCP1 Belgian rabbit New Zealand rabbit California rabbit polymorphism |
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