多倍体无籽罗汉果及其亲本遗传背景的ISSR分析

 应用ISSR分子标记探讨多倍体无籽罗汉果及其亲本材料的遗传背景。结果发现：多倍体无籽罗汉果及其亲本的相似性系数为0.6399 ~ 0.8566，F₁代与四倍体母本之间的平均相似性系数高于其与二倍体父本之间的相似性系数，说明子代从母本继承的遗传物质更多，遗传上倾向母本。且F₁代与亲本之间的平均遗传相似性系数大于或小于亲本之间，随亲本的组合和相应的F₁代而定；结合聚类图和双变量主坐标分析，可知：基本上子代和母本排列并聚在一起，而且父本、母本、子代彼此之间距离相对较近地聚在一起；因此还是体现了“子似亲”的遗传现象。

ISSR Analysis of Genetic Background of Polyploidy Seedless Siraitia grosvenorii and Their Parents

In this study,the genetic background of polyploidy seedless Siraitia grosvenorii and their parents were revealed by ISSR(Inter simple sequence repeats)molecular marker technique.The results showed that the genetic similarity coefficient between two individuals was from 0.6399 to 0.8566,and the average coefficient between F1 generation and tetraploid female parent was higher than that between F1 generation and diploid male parent,which showed that the F1 generation got more genetic substance from female parent than that from male parent,and the genetic phenomenon showed F1 generation was apt to female parent.And the average coefficient between F1 generation and parents was higher or lower than that between parents.According to dendrogram analysis and double principal coordinate analysis,filial generation and female parent arrayed and clustered together,and if genetic distance of male parent,female parent and filial generation was near,they would array together too.So,in general,the genetic phenomenon embodied"filial generation was similar to parents".