5–氮杂胞苷对白菜幼苗DNA 甲基化和耐热性的影响

 研究了不同浓度5–氮杂胞苷（5-azaC）处理白菜‘夏帝’和‘冬赏味’品种的种子，对其幼苗甲基化和耐热性的影响。结果表明：不同浓度5-azaC 溶液处理可显著降低幼苗DNA 甲基化水平，植株形态发育未见异常。0.1 ~ 0.2 mol · L^-1 5-azaC 处理可减缓幼苗在高温胁迫下的生长量、POD 活性和蛋白质含量的降低幅度，同时降低MDA 含量和细胞膜透性。

The Effects of 5-azacytidine on DNA Methylation and Heat Tolerance of Seedlings of Non-heading Chinese Cabbage

Segregating population consisting of BC1F1,BC2F1 and F2 were generated by crossing the resistant inbred line PI482398male parent,containing gene the Gsb-4 resistant to gummy stem blight (GSB)]with the susceptible inbred line‘Baipicui’(female parent). The resistance of segregating population and parental lines were evaluated by inoculating spore isolates of GSB at seeding stage. The results indicated that resistance to GSB in PI482398 is controlled by a single dominant gene. The bulked segregant analysis(BSA)was employed to screen the resistant and susceptible DNA pools with 89 pairs of SSR primers as tool. An unique 120 bp fragment was amplified in resistant materials with the primer CMTA170a. The polymorphic patterns of CMTA170a in the 118 individuals of F2 were scored and then calculated by the software of MAPMAKER/Exp version 3.0 b. The results showed that the genetic distance between CMTA170a and Gsb-4 is 5.14 cM.