| 亚洲百合与青岛百合杂交及其 FISH 快速鉴定 |
| |
| 引用本文： | 任贵玲,张线线,周树军.亚洲百合与青岛百合杂交及其 FISH 快速鉴定[J].园艺学报,2012,39(3):588-592. |
| |
| 作者姓名： | 任贵玲 张线线 周树军 |
| |
| 作者单位： | （浙江大学农业与生物技术学院园艺系，农业部园艺植物生长发育与品质调控重点开放实验室，杭州 310058） |
| |
| 基金项目： | 国家自然科学基金项目，教育部留学回国人员科研启动基金项目，浙江省钱江人才项目 |
| |
| 摘 要： | 利用切柱头授粉和胚抢救技术获得亚洲百合‘Petit Brigitte’× 青岛百合的14 个杂交后代，以45S rDNA 为探针对亲本和其中4 个后代进行了荧光原位杂交（FISH）分析，结果表明，亲本和后代都为二倍体（2n = 2x = 24）；‘Petit Brigitte’有10 个45S rDNA 位点，分别位于1、2、4、5 和7 号染色体上；青岛百合有8 个45S rDNA 位点，分别位于3、4、5 和10 号染色体上；4 个杂种后代有9 个45S rDNA位点，分别源于‘Petit Brigitte’和青岛百合。
|
| 关 键 词： | 百合 远缘杂交 rDNA 荧光原位杂交 |
Hybridization and Identification Between Asiatic Lily and Lilium tsingtauense Using FISH |
| |
| REN Gui-ling,ZHANG Xian-xian,and ZHOU Shu-jun.Hybridization and Identification Between Asiatic Lily and Lilium tsingtauense Using FISH[J].Acta Horticulturae Sinica,2012,39(3):588-592. |
| |
| Authors: | REN Gui-ling ZHANG Xian-xian and ZHOU Shu-jun |
| |
| Institution: | （The Key Laboratory of Horticultural Plant Growth，Development and Quality Improvement，the Ministry of Agriculture of the People’s Republic of China；Department of Horticulture，College of Agriculture and Biotechnology，Zhejiang University，Hangzhou 310058，China） |
| |
| Abstract: | Hybridization between Asiatic lily‘Petit Brigitte’× Lilium tsingtauense was conducted with cut style pollination and embryo rescue. Fourteen progenies were obtained,and the parents and 4 progenies were analyzed using FISH with 45S rDNA as probe. The results showed that all of them are diploid(2n = 2x = 24);‘Petit Brigitte’has 10 loci of 45S rDNA distributed on chromosome CH-1,CH-2,CH-5 and CH-7 and Lilium tsingtauense has 8 on CH-3,CH-4,CH-5 and CH-10 respectively,2n = 2x = 24 for Lilium tsingtauense and 2n = 2x = 24 for hybrids,respectively;all four progenies have 9 loci of 45S rDNA,unambiguously,coming from‘Petit Brigitte’and Lilium tsingtauense respectively. |
| |
| Keywords: | Lilium distant hybridization rDNA fluorescence in situ hybridization(FISH) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
