Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated
from migratory wild geese in Japan |
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| Authors: | Eiji YAMAMOTO Hiroshi ITO Yukiko TOMIOKA Toshihiro ITO |
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| Institution: | 1)United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753–8515, Japan;2)Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, 4–101 Koyama-Minami, Tottori 680–8553, Japan;3)Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University, 4–101 Koyama-Minami, Tottori 680–8553, Japan |
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| Abstract: | An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was
biologically, serologically and genetically characterized. APMV/Shimane67 showed typical
paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test,
antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and
vice versa. The fusion (F) protein gene of APMV/Shimane67 contained
1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids.
The cleavage site of F protein contained a pair of single basic amino
acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene
of APMV/Shimane67 had relatively low identities (42.9–62.7% and 28.9–67.3%, respectively)
with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to
NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest
that APMV/Shimane67 is a new APMV serotype, APMV-13. |
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| Keywords: | APMV avian paramyxovirus F gene phylogenetic analysis serotype |
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