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南荻MlNAC2基因差异表达与耐盐生理响应的相关性分析
引用本文:万婷,段钧译,李蒙,陈智勇. 南荻MlNAC2基因差异表达与耐盐生理响应的相关性分析[J]. 草地学报, 2021, 29(12): 2685-2693. DOI: 10.11733/j.issn.1007-0435.2021.12.007
作者姓名:万婷  段钧译  李蒙  陈智勇
作者单位:1. 湖南农业大学, 湖南 长沙 410128;2. 芒属植物生态应用技术湖南省工程实验室, 湖南 长沙 410128;3. 国家能源非粮生物质原料研发中心湖南分中心, 湖南 长沙 410128;4. 湖南省蚕桑科学研究所, 湖南 长沙 410127
基金项目:国家自然科学基金面上项目(31471557);国家自然科学基金青年项目(32000260);湖湘高层次人才聚集工程(2019RS1051);长沙市科技局国际合作重点项目(kq1901031)资助
摘    要:为探究南荻(Miscanthus lutarioriparius)耐盐生理及其与基因表达的相关性,采用0%,0.2%,0.5%,0.8%浓度的NaCl处理奇岗和不同基因型南荻,测量NaCl溶液胁迫下的MlNAC2基因相对表达量和6个耐盐指标,并对二者之间的相关性进行分析.结果 显示,南荻MlNAC2基因的表达量变化在不...

关 键 词:盐胁迫  南荻  生理响应  MlNAC2基因  相关性分析
收稿时间:2021-04-26

Correlation Between MlNAC2 Gene Differential Expression and Salt-resistance Physiological Response in Miscanthus lutarioriparius Polyploid
WAN Ting,DUAN Jun-yi,LI Meng,CHEN Zhi-yong. Correlation Between MlNAC2 Gene Differential Expression and Salt-resistance Physiological Response in Miscanthus lutarioriparius Polyploid[J]. Acta Agrestia Sinica, 2021, 29(12): 2685-2693. DOI: 10.11733/j.issn.1007-0435.2021.12.007
Authors:WAN Ting  DUAN Jun-yi  LI Meng  CHEN Zhi-yong
Affiliation:1. Hunan Agricultural University, Changsha, Hunan Province 410128, China;2. Miscanthus Ecological Application Technology, Hunan Engineering Laboratory, Changsha, Hunan Province 410128, China;3. Hunan Branch, National Energy R&D Center for Non-food Biomass, Hunan Agricultural University, Changsha, Hunan Province 410128, China;4. The Sericultural Research Institute of Hunan Province, Changsha, Hunan Province 410127, China
Abstract:In this study,different Miscanthus lutarioriparius genotypes and Miscanthus×giganteus were used as test materials to explore the correlation between physiological responses and gene expression under salt stress. Those materials were treated with 0%,0.2%,0.5%,0.8% NaCl solutions individually. The expression of MlNAC2 gene and 6 physiological indexes were detected,and the correlation was analyzed. The results showed that the changes of MlNAC2 gene varied greatly among different materials,L2,L6,L9,and P1 increased more than 10 times while L1,L5,L8,and L10 changed less than 3 times. All materials were significantly different from the control in chlorophyll content,net photosynthetic rate,stomatal conductance,and proline,while some materials were not significantly different from the control in malondialdehyde content and superoxide dismutase activity. Correlation analysis showed that a series of photosynthetic factors referring to chlorophyll content,net photosynthetic rate,and stomatal conductance were related to MlNAC2 gene mostly. The results of this study can provide a reference for exploring the relationship between physiological response and molecular mechanism.
Keywords:Salt stress  Miscanthus lutarioriparius  Physiological response  MlNAC2 gene  Correlation analysis  
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