| Tagging and Utilization Bruchid Resistance Gene Using PCR Markers in Mungbean |
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| 引用本文： | CHENG Xu-zhen WANG Su-hua WU Shao-yu ZHOU Ji-hong WANG Shu-min Charles Y Yang. Tagging and Utilization Bruchid Resistance Gene Using PCR Markers in Mungbean[J]. 中国农业科学(英文版), 2005, 4(8): 579-583 |
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| 作者姓名： | CHENG Xu-zhen WANG Su-hua WU Shao-yu ZHOU Ji-hong WANG Shu-min Charles Y Yang |
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| 作者单位： | [1]Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beiiing 10081, P.R.China [2]Beijing Municipal Seeds Company, Beijing 100088, P.R.China [3]Asian Regional Center, Asian Vegetable Research and Development Center, Bangkok 10903, Thailand |
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| 摘 要： |
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| 关 键 词： | 绿豆 PCR分析 抗性基因 DNA |
Tagging and Utilization Bruchid Resistance Gene Using PCR Markers in Mungbean |
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| CHENG Xu-zhen,WANG Su-hua,WU Shao-yu,ZHOU Ji-hong,WANG Shu-min,Charles Y Yang. Tagging and Utilization Bruchid Resistance Gene Using PCR Markers in Mungbean[J]. 《Agricultural Sciences in China》, 2005, 4(8): 579-583 |
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| Authors: | CHENG Xu-zhen WANG Su-hua WU Shao-yu ZHOU Ji-hong WANG Shu-min Charles Y Yang |
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| Abstract: | Sixteen mungbean lines were analyzed using 56 random primers. Different DNA bands were detected between Bruchid resistant lines and susceptible lines. According to the cluster results, the 16 lines can be divided into four groups, including brucid resistant wild types, resistant cultivated lines, resistant progenies and a mixed group. BSA method was used to identify DNA markers that related with bruchid resistant gene by using resistant line and susceptible line and their F2 progeny. One codominant marker was identified, which generated a fragment of 1.79 kb in resistant lines and 1.03 kb in susceptible lines. Finally, this codominant marker was considered to be tightly linked with bruchid resistant gene and could be useful in resistant germplasm identification and marker-assisted selection. |
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| Keywords: | Mungbean [Vigna radiata(L.) Wilclzek] Bruchid resistant gene DNA marker |
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