| 农杆菌介导草地早熟禾转化条件的优化 |
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| 引用本文： | 王娟.农杆菌介导草地早熟禾转化条件的优化[J].安徽农业科学,2010,38(1):58-59,62. |
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| 作者姓名： | 王娟 |
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| 作者单位： | 宁夏大学生命科学学院,宁夏银川,750021 |
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| 摘 要： | 目的]提高草地早熟禾的转化频率。方法]以草地早熟禾的丛生芽芽尖为转化受体，研究了影响农杆菌转化草地早熟禾频率的有关因素。结果]农杆菌悬浮液浓度OD600=0．6，并添加0．1mmoL／L乙酰丁香酮，在50kPa真空处理5min，获得的抗性芽比例较高，最大达15．19％。结论]农杆菌悬浮液浓度、真空渗透处理及乙酰丁香酮的诱导均能影响草地早熟禾的转化频率。
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| 关 键 词： | 草地早熟禾 农杆菌 转化 |
Optimization of Conditions Agrobacterium-mediated Transformation in Kentucky Bluegrass |
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| WANG Juan.Optimization of Conditions Agrobacterium-mediated Transformation in Kentucky Bluegrass[J].Journal of Anhui Agricultural Sciences,2010,38(1):58-59,62. |
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| Authors: | WANG Juan |
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| Institution: | School of Life Sciences;Ningxia University;Yinchuan;Ningxia 750021 |
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| Abstract: | Objective]The study aimed to enhance the transformation frequency of Kentucky bluegrass.Method]With shoot tips of multiple bud multiple as transformation target,the factors which influenced transformation of Kentucky bluegrass mediated by Agrobacterium tumefaciens were studied.Result]Higher percentage of resistant buds(hygromycin-resistant buds / infected buds) was obtained under the following conditions: shoot tips were infected in Agrobacterium suspension of OD600=0.6 supplemented with 0.1 mmol/L aceto... |
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| Keywords: | Kentucky bluegrass Agrobacterium tumefaciens Transformation |
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