马尾松亲本遗传距离与子代生长性状相关性分析

利用ISSR标记分析了马尾松测交亲本遗传距离与11年生子代生长性状的相关性,发现亲本遗传距离在0.158 - 0.657范围时与子代各生长性状的相关性较低,而当遗传距离在0.264 - 0.529区间时与子代胸径、材积性状的相关系数分别为0.493和0.469, 一元线性回归拟合效果均达显著水平,表明随着亲本间遗传距离的增大,马尾松胸径、材积等性状呈显著线性增加。当遗传距离位于0.357 - 0.529区间时,遗传距离与生长性状的相关系数比其在0.264 - 0.529区间时的相关系数增加,与材积性状相关系数为0.635。本研究证实马尾松亲本间ISSR分子遗传差异对于子代生长预测和杂交亲本选配具有一定参考价值,但由于相关系数较小,尚不能提供决定性作用,而在本研究基础上建立更完善的分子标记实验模型,将利于有效进行马尾松杂种优势辅助聚合。

Relationship between Hybrid Performance and Genetic Distances amongPinus massoniana Clones Based on ISSR Molecular Marker

ISSR (intro-simple sequence repeat) were employed to detect the GD (genetic distance) among 13 Pinus massoniana parent clones and the relationship between GD and hybrid performance. There was significant positive correlation between GD and hybrid performance on condition when GD ranged 0.264 - 0.529, and the relationship between GD and growth traits including height, DBH and volume index followed a linear equation, implying the potential value of ISSR in the molecular assisting selection (MAS) of Pinus massoniana. The correlation coefficient was larger when GD ranged between 0.357 - 0.529 than between 0.264 - 0.529. However, the correlation coefficient was lower than 0.700, and more effective model is needed to achieve more efficient molecular assisting selection for Pinus massoniana.