| 苦碟子总黄酮提取工艺研究 |
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| 引用本文： | 陈英红,姜翔之,罗浩铭,姜瑞芝. 苦碟子总黄酮提取工艺研究[J]. 特产研究, 2011, 33(1): 29-30,54 |
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| 作者姓名： | 陈英红 姜翔之 罗浩铭 姜瑞芝 |
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| 作者单位： | 1. 吉林省中医药科学院,长春,130012
2. 吉林大学中日联谊医院药剂科,长春,130051 |
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| 基金项目： | 国家十一五科技重大专项 |
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| 摘 要： | 探索从苦碟子中提取总黄酮的最佳工艺。采用分光光度法测定总黄酮含量，通过单因素试验分析了乙醇浓度、回流时间、提取次数、溶剂用量4个主要因素对苦碟子总黄酮提取率的影响，并在单因素试验的基础上通过正交设计法优化了苦碟子总黄酮提取工艺条件。影响苦碟子总黄酮的主次因素为：乙醇浓度〉回流时间〉溶剂用量〉提取次数。苦碟子总黄酮最佳提取工艺为：10倍量60％乙醇回流提取2次，每次2h。
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| 关 键 词： | 苦碟子 总黄酮 正交设计 |
Study on the Extraction Process of Total Flavonoids from Ixeris Sonchifolia (Bge.) Hance |
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| CHEN Ying-hong,JIANG Xiang-zhi,LUO Hao-ming,JIANG Rui-zhi. Study on the Extraction Process of Total Flavonoids from Ixeris Sonchifolia (Bge.) Hance[J]. Special Wild Economic Animal and Plant Research, 2011, 33(1): 29-30,54 |
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| Authors: | CHEN Ying-hong JIANG Xiang-zhi LUO Hao-ming JIANG Rui-zhi |
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| Affiliation: | 1 (1.Jilin Academy of Traditional Chinese Medicine and Material Medical Sciences,Changchun 130012,China; 2.Japan China Union Hospital,JiLin University,Changchun 130051,China) |
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| Abstract: | To study and optimize the extraction procedure for total flavonoids from Ixeris Sonchifolia(Bge.) Hance. Spectrophotometry was adopted to determine content of total flavonoids in Ixeris Sonchifolia(Bge.) Hance.Effects of alcohol concentration,extraction duration,extraction times,volume of alcohol on extraction rate of total flavonoids were analyzed using single-factor test.Based on this,the extraction technological conditions of total flavonoids from Ixeris Sonchifolia(Bge.) Hance were optimized through orthogonal design method.The factors that influenced the extraction rate were sorted as follows:the concentration of ethanol>extraction time>volume of ethanol>heated times.The optimal process of the extraction technology which obtained through orthogonal design is:60%ethanol,ten-fold solvent,two times extraction,two hours per extraction time. |
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| Keywords: | Ixeris Sonchifolia(Bge.) Hance Total flavonoids Orthogonal design |
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