| 杨树热激转录因子HsfA1d的克隆、表达及单核苷酸多态性分析 |
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| 引用本文： | 赵杏,郭琦,陈清清,李百炼,张德强. 杨树热激转录因子HsfA1d的克隆、表达及单核苷酸多态性分析[J]. 林业科学, 2011, 0(7) |
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| 作者姓名： | 赵杏 郭琦 陈清清 李百炼 张德强 |
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| 作者单位： | 北京林业大学林木育种国家工程实验室林木花卉遗传育种教育部重点实验室; |
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| 基金项目： | 国家林业公益性行业科研专项经费项目(201004009); 教育部新世纪优秀人才支持计划专项基金(NCET-07-0084) |
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| 摘 要： | 利用RT-PCR方法,首次由小叶杨受热激胁迫后构建的cDNA文库中扩增获得一热激转录因子HsfA1d cDNA克隆,测序结果表明克隆的PsHsfA1d cDNA片段总长为2036bp,基因内部含有完整的开放阅读框架,大小为1449bp,可编码长度为482个氨基酸残基的蛋白质,所推导的蛋白质氨基酸序列在HSF DBD结构功能域与拟南芥AtHsfA1d和水稻OsHsfA1蛋白的相似性分别为86.3%和87.4%。组织特异性Realtime-PCR结果显示,PsHsfA1d主要在杨树叶片和根部组织中高丰度表达。非生物胁迫、激素及糖诱导表达表明,PsHsfA1d不仅受高温、干旱与盐诱导表达,还受到激素中GA_3与ABA,及葡萄糖与蔗糖信号的上调表达。组合利用MEGA4.0和DnaSP4.50.7软件对小叶杨36株基因型个体的PsHsfA1d序列进行比对和分析,共检测到207个单核苷酸多态性(SNP)位点,SNP频率为1/16bp,多样性指数π为0.00772。在编码区域,共检测到69个SNP位点,其中37个为同义突变,31个为错义突变,1个为无义突变;非同义突变与同义突变的比率0.132<1,推测在小叶杨物种演化过程...
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| 关 键 词： | 小叶杨 HsfA1d基因 热胁迫 诱导表达 单核苷酸多样性 |
Isolation, Expression and Single Nucleotide Polymorphisms Analysis of Heat Shock Transcription Factor HsfA1d in Populus |
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| Zhao Xing Guo Qi Chen Qingqing Li Bailian Zhang Deqiang. Isolation, Expression and Single Nucleotide Polymorphisms Analysis of Heat Shock Transcription Factor HsfA1d in Populus[J]. Scientia Silvae Sinicae, 2011, 0(7) |
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| Authors: | Zhao Xing Guo Qi Chen Qingqing Li Bailian Zhang Deqiang |
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| Affiliation: | Zhao Xing Guo Qi Chen Qingqing Li Bailian Zhang Deqiang (National Engineering Laboratory for Tree Breeding Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education Beijing Forestry University Beijing 100083) |
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| Abstract: | A cDNA clone encoding HsfA1d was isolated from a cDNA library prepared under heat shock stress in Populus simonii by the RT-PCR method. The cDNA of PsHsfA1d is 2 036 bp in length with an open reading frame (ORF, 1 449 bp in length) which is capable of encoding a protein of 482 amino acids. The deduced protein sequence of the PsHsfA1d shares 86.3%, and 87.4% identity with functional domain of HSF DBD of Arabidopsis thaliana AtHsfA1d, and Oryza sativa OsHsfA1, respectively. Tissue differential expression with... |
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| Keywords: | Populus simonii HsfA1d gene heat shock stress inducible expression single nucleotide polymorphisms |
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