| 利用中间偃麦草抗病基因同源序列分离黄矮病抗性候选基因克隆 |
| |
| 引用本文： | 张增艳,许景升,刘耀光,王晓萍,林志珊,辛志勇.利用中间偃麦草抗病基因同源序列分离黄矮病抗性候选基因克隆[J].作物学报,2004,30(3):189-195. |
| |
| 作者姓名： | 张增艳 许景升 刘耀光 王晓萍 林志珊 辛志勇 |
| |
| 作者单位： | 中国农业科学院作物育种栽培研究所，农业部作物遗传育种重点实验室，北京100081 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 根据已克隆植物抗病（R）基因编码蛋白质的保守结构设计简并引物，利用同源序列法PCR扩增、克隆到9个具有开放阅读框的中间偃麦草R基因同源片段（Resistance Gene Analogs，RGAs）。利用抗黄矮病材料(含Bdv2)、感黄矮病材料（无Bdv2）进行RFLP分析，筛选到1个NBS类RGA序列TirgaZ1与Bdv2连锁。根据TirgaZ1的序列重新设计1对引
|
| 关 键 词： | 中间偃麦草 黄矮病抗性 抗病基因同源序列 可转化人工染色体（TAC） 克隆池PCR |
| 收稿时间： | 2003-02-26 |
| 修稿时间： | 2003年2月26日 |
Isolation of Resistance Gene Candidates by a Resistance Gene Analog of Thinopyrum intermedium and Pooled-PCR |
| |
| ZHANG Zeng-Yan,XU Jing-Sheng,LIU Yao-Guang,WANG Xiao-Ping,LIN Zhi-Shan,XIN Zhi-Yong.Isolation of Resistance Gene Candidates by a Resistance Gene Analog of Thinopyrum intermedium and Pooled-PCR[J].Acta Agronomica Sinica,2004,30(3):189-195. |
| |
| Authors: | ZHANG Zeng-Yan XU Jing-Sheng LIU Yao-Guang WANG Xiao-Ping LIN Zhi-Shan XIN Zhi-Yong |
| |
| Institution: | Key Lab of Crop Genetics and Breeding，Ministry of Agriculture，Institute of Crop Breeding and Cultivation，CAAS，Beijing 100081 |
| |
| Abstract: | Based on the known R gene conserved domains, 10 pairs of degenerate primers were synthesized and used to amplify genomic DNAs of Th. intermedium and translocation line HW642. The PCR products of Th. intermedium were excised and cloned. Clones were sequenced and compared to sequence homolog with gene |
| |
| Keywords: | Thinopyrum intermedium Resistance to barley yellow dwarf virus Resistance gene analog(RGA) Transformation-competent artificial chromosome(TAC) Pooled-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
