白首乌的离体再生与快速繁殖

目的]加快白首乌种苗生产速度及实现其生产的现代化。方法]以带侧芽白首乌茎段为外植体,采用MS为基本培养基,附加不同植物生产调节剂进行试验。结果]结果表明,适宜于白首乌的初代培养基为:1/2 MS+6-BA 0.50 mg/L+NAA 0.05 mg/L;增殖培养基:MS+6-BA 0.20 mg/L+NAA 0.10 mg/L;生根培养基:1/2 MS+IBA 0.20 mg/L。移栽成活率达100%。结论]采用白首乌的茎段作为外植体,利用组织培养的方法能实现种苗的快速工厂化。

Study on in vitro Culture and Rapid Propagation of Cynanchum auriculatum

Objective]The purpose aimed to speed up seedling production of Cynanchum auriculatum and its modernization.Method] With tissue culture method,primary culture of explants,bud multiplication and their rooting culture were conducted by medium treated with different hormones.Result]The results indicated that the appropriate medium for different culture stages were 1/2 MS+6-BA 0.50 mg/L+ NAA 0.05 mg/L in primary culture,MS+6-BA 0.20 mg/L+NAA 0.10 mg/L for bud multiplication,1/2 MS+IBA 0.20 mg/L in rooting.The survival rate of transplanting could reach 100%.Conclusion] Applying stem as explants,high frequency propagation of seedlings can be achieved with plant tissue culture in Cynanchum auriculatum.