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| DNA指纹技术在近交系小鼠遗传检测中的应用研究 | |
| 引用本文： | 段天林,刘英,王芳,张文慧.DNA指纹技术在近交系小鼠遗传检测中的应用研究[J].甘肃农业大学学报,2009,44(2). |
| 作者姓名： | 段天林 刘英 王芳 张文慧 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070;兰州大学医学实验中心,甘肃,兰州,730000 2. 甘肃农业大学动物医学院,甘肃,兰州,730070 3. 兰州大学医学实验中心,甘肃,兰州,730000 |
| 摘 要： | 采用生物素标记的(GGAT)4寡核苷酸探针对C57BL/6J、BALB/c和DBA/2三种近交系小鼠进行了DNA指纹图分析.结果表明:(GGAT)4寡核苷酸探针对上述3种近交系小鼠产生的DNA指纹图的图带数均为10-12条,具有良好的多态性,品系内平均DNA指纹图的相似系数在0.92~1.00的范围内,具有相同指纹图的概率均在0.40以上,极显著地高于品系间的相似系数(0.21~0.39)和相同指纹图的概率(P<3.10×10-7),说明(GGAT)4寡核苷酸探针可用于制作近交系小鼠的DNA指纹图,以对其进行遗传检测. |
| 关 键 词： | 近交系小鼠 DNA指纹技术 遗传检测 |
Application of DNA fingerprinting in genetic monitoring of inbred strain mice |
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| DUAN Tian-lin,LIU Ying,WANG Fang,ZHANG Wen-hui.Application of DNA fingerprinting in genetic monitoring of inbred strain mice[J].Journal of Gansu Agricultural University,2009,44(2). | |
| Authors: | DUAN Tian-lin LIU Ying WANG Fang ZHANG Wen-hui |
| Institution: | DUAN Tian-lin1,2,LIU Ying1,WANG Fang2,ZHANG Wen-hui2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China,2.Center of Medical Laboratory,Lanzhou University,Lanzhou 730000,China) |
| Abstract: | The objective of this paper is to evaluate DNA fingerprinting of BALB/c,C57BL/6J and DBA/2 inbred strain mice with the biotin-labeled(GGAT)4 oligonucleotide probe.There were 10-12 distinguishable bands in every DNA fingerprinting map and the oligonucleotide probe(GGAT)4 had an excellent polymorphism in 3 inbred strain mice.The similarity coefficient(x) within breeds was 0.92～1.00,and the probability of an identical DNA fingerprinting(P) was above 0.40.These two indices within breed were significantly higher than those between breeds(x=0.21～0.39,P<3.10×10-7).These results showed that the oligonucleotide probe(GGAT)4 can be used in DNA fingerprinting map for genetic monitoring of inbred strain mice. |
| Keywords: | inbred strain mice DNA fingerprinting genetic monitoring |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |