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Population structure of South African and Australian Pyrenophora teres isolates |
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| Authors: | A Lehmensiek A E Bester‐van der Merwe M W Sutherland G Platz W M Kriel G F Potgieter R Prins |
| Institution: | 1. Centre for Systems Biology, University of Southern Queensland, Toowoomba, QLD 4350, Australia;2. Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Stellenbosch 7600, South Africa;3. Department of Employment, Economic Development and Innovation, Queensland Primary Industries and Fisheries, Hermitage Research Station, Warwick, QLD 4370, Australia;4. Department of Plant Sciences, University of Free State, Bloemfontein 9300, South Africa;5. South African Barley Breeding Institute, PO Box 27, Caledon 7230, South Africa;6. CenGen (Pty) Ltd, 78 Fairbairn Street, Worcester 6850, South Africa |
| Abstract: | There are two recognized forms of the disease net blotch of barley: the net form caused by Pyrenophora teres f. teres (PTT) and the spot form caused by P. teres f. maculata (PTM). In this study, amplified fragment length polymorphism analysis was used to investigate the genetic diversity and population structure of 60 PTT and 64 PTM isolates collected across Australia (66 isolates) and in the south‐western Cape of South Africa (58 isolates). For comparison, P. tritici‐repentis, Exserohilum rostratum and Bipolaris sorokiniana samples were also included in the analyses. Both distance‐ and model‐based cluster analyses separated the PTT and PTM isolates into two strongly divergent genetic groups. Significant variation was observed both among the South African and Australian populations of PTT and PTM and among sampling locations for the PTT samples. Results suggest that sexual reproduction between the two forms is unlikely and that reproduction within the PTT and PTM groups occurs mainly asexually. |
| Keywords: | barley form‐specific markers genetic variation Hordeum vulgare Pyrenophora teres f maculata Pyrenophora teres f teres |