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Bioinformatics‐assisted characterization of the ITS1‐5·8S‐ITS2 segments of nuclear rRNA gene clusters,and its exploitation in molecular diagnostics of European crop‐parasitic nematodes of the genus Ditylenchus
Authors:M Marek  M Zouhar  O Douda  J Mazakova  P Rysanek
Institution:1. Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 957, 165 21 Prague 6 – Suchdol;2. Present address: Department of Bioprocess Development, Généthon, 1bis, rue de l’Internationale – 91002 évry Cédex, France.;3. Laboratory of Nematology, Crop Research Institute, Drnovska 507, 161 06 Prague 6 – Ruzyne, Czech Republic
Abstract:
Keywords:Ditylenchus destructor  Ditylenchus dipsaci  internal transcribed spacers  ITS2 secondary structure  multiplex PCR  ribosomal DNA
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