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| 欧洲榛微卫星对我国榛属种质资源的分析 | |
| 引用本文： | 程丽莉,黄武刚,周志军,刘建立,王艳梅,苏淑钗,翟明普.欧洲榛微卫星对我国榛属种质资源的分析[J].林业科学,2009,45(2). |
| 作者姓名： | 程丽莉 黄武刚 周志军 刘建立 王艳梅 苏淑钗 翟明普 |
| 作者单位： | 1. 北京市农林科学院林业果树研究所,北京,100093 2. 北京市昌平林业局,北京,102200 3. 北京林业大学,北京,100083 |
| 基金项目： | 北京市留学人员科技活动择优项目，北京市优秀人才培养资助项目 |
| 摘 要： | 利用多态性高、重复性好的11对欧洲榛微卫星引物对榛属6个近缘种的34个DNA样本进行扩增,共获得81个等位基因,每个座位的等位基因数在4～13之间,平均数目为7.36个,位点的多态信息含量(PIC)介于0.47～0.86,平均为0.73.PCR扩增产物的DNA测序证明欧洲榛SSR基因座位中微卫星序列在6个种内的保守性,而在CAC C28位点,一个被忽略的短三碱基重复序列也表现出长度多态性.另外,对于具有商业潜力的平榛、毛榛、川榛3个种的遗传多样性分析表明,平榛的遗传多样性最高,为0.59.研究结果表明欧洲榛微卫星是用于榛属资源保护、品种改良以及种问遗传图谱构建的有力工具. |
| 关 键 词： | 榛属 微卫星 多态性 DNA测序 |
Genetic Diversity of Six Corylus Species in China Detected with Microsatellite Isolated from Corylus avellana |
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| Cheng Lili,Huang Wugang,Zhou Zhijun,Liu Jianli,Wang Yanmei,Su Shuchai,Zhai Mingpu.Genetic Diversity of Six Corylus Species in China Detected with Microsatellite Isolated from Corylus avellana[J].Scientia Silvae Sinicae,2009,45(2). | |
| Authors: | Cheng Lili Huang Wugang Zhou Zhijun Liu Jianli Wang Yanmei Su Shuchai Zhai Mingpu |
| Institution: | 1.Beijing Institute of Forestry and Pomology;Beijing Academy of Agricultural and Forestry Sciences Beijing 100093;2.Changping District Forest Department Beijing 102200;3.Beijing Forestry University Beijing 100083 |
| Abstract: | Diversity of 6 Corylus species distributed in China was analyzed by 11 SSR markers isolated from C.avellana.A total of 81 alleles were amplified by 11 high informative SSR primer pairs from 34 DNA templates of the accessions tested.Number of alleles per locus ranged from 4 to 13 with an average value of 7.36.Average value of polymorphic information content(PIC) was 0.73,ranged from 0.47 to 0.86.Sequencing the PCR amplified DNA showed there existed microsatellites of C.avellana in 6 Corylus species.Other sho... |
| Keywords: | Corylus SSR marker polymorphism DNA sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |