| PRRSV新疆分离株的ORF5序列分析与同源性比较 |
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| 引用本文： | 苏贵成,盛卓君,马素贞,胡峻,王薇,袁江玲,黄家雨,简子健. PRRSV新疆分离株的ORF5序列分析与同源性比较[J]. 新疆农业大学学报, 2008, 31(6) |
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| 作者姓名： | 苏贵成 盛卓君 马素贞 胡峻 王薇 袁江玲 黄家雨 简子健 |
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| 作者单位： | 1. 新疆农业大学 动物医学学院,乌鲁木齐,830052
2. 新疆动物卫生监督所,乌鲁木齐,830063 |
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| 摘 要： | 采用RT-PCR技术,分别对PRRSV新疆分离株XJ-a,XJ-b,XJ-c的ORF5片段的ORF5基因进行扩增,获得长度为603 bp的特异性目片段.ORF5片段序列分析表明,新疆3个分离株的ORF5基因与欧洲型PRRSV毒株序列核苷酸的同源性为56.1%～56.4%,与标准美洲型PRRSV毒株序列的核苷酸同源性达到86.6%～87.2%,与国内部分省市流行株的同源性在96.0%～99.2%.PRRSV新疆分离株属于北美洲型PRRSV的变异毒株,属同一亚型,毒株间也存在一定的差异,具有高度致病性的生物学特征.
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 序列分析 同源性比较 |
Comparison of the Homology and Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Xinjiang |
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| SU Gui-cheng,SHENG Zhuo-jun,MA Su-zhen,HU Jun,WANG Wei,YUAN Jiang-ling,HUANG Jia-yu,JIAN Zi-jian. Comparison of the Homology and Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Xinjiang[J]. Journal of Xinjiang Agricultural University, 2008, 31(6) |
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| Authors: | SU Gui-cheng SHENG Zhuo-jun MA Su-zhen HU Jun WANG Wei YUAN Jiang-ling HUANG Jia-yu JIAN Zi-jian |
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| Affiliation: | SU Gui-cheng1,SHENG Zhuo-jun2,MA Su-zhen1,HU Jun2,WANG Wei2,YUAN Jiang-ling1,HUANG Jia-yu1,JIAN Zi-jian1 (1.College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China,2.Xinjiang Animal Health Supervision Station,Urumqi 830063,China) |
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| Abstract: | The ORF5 genes of strains XJ-a,XJ-b,XJ-c isolated from Xinjiang were amplified and the special 603bp fragments were obtained by RT-PCR.In the sequence analysis of ORF5 gene of Xinjiang PRRSV isolates,comparing with the prototype PRRSV from Europe,the Northern America and the prevalent strains from some provinces of China,the homology were from 56.1% to 56.4%,from 86.6% to 87.2% and from 96.0% to 99.2% respectively.The results were shown that Xinjiang PRRSV isolates belonged to variant strains of Northern Am... |
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| Keywords: | PRRSV sequence analysis homologous comparison |
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