金边瑞香插穗形态、POD与可溶性蛋白含量变化研究

分析金边瑞香扦插生根过程中插穗形态变化、POD活性和可溶性蛋白含量的变化规律与生根的关系,为其生产和生根机理研究提供理论依据。结果表明,600 mg.L-1的NAA溶液能有效抑制金边瑞香插穗基部变褐和腐烂,促进愈伤和生根的发生,最高生根率达86.8%,生根率与皮层开裂呈正相关。生根过程中,插穗体内POD活性总体呈"升高-降低-升高"趋势,最高峰值较CK提早了8 d,与愈伤高峰出现一致;可溶性蛋白总体呈"降低-升高"趋势,各处理下降的速度较CK要快。

Study On morphology characteristics,POD activity and soluble proteins content on cutting of Daphne odora var.marginata Mak

The relationship of Daphne odora var.marginata Mak among morphology,POD activity,soluble proteins and cuttings rooting were analyzed in order to provide a theoretical basis for production and rooting mechanism.The results showed that 600 mg·L-1 of NAA colutin could effectively inhibit Daphne odora var.marginata cuttings browning and decay,and promote callus and rooting.The rooting rate was more than 86.8%.There was positively relationship between rooting rate and cortex cracking.During the rooting process,POD activity was"higher-lower-higher".Peak of POD activity was eight days ahead of CK,which was consistent with callus peak.Soluble protein was the trend of"lower-higher"and the decline speed of each treatment was faster than one of CK.