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| 百合抗尖孢镰刀菌无性系的离体筛选 | |
| 引用本文： | 丁丁. 百合抗尖孢镰刀菌无性系的离体筛选[J]. 湖南农业大学学报(自然科学版), 2011, 37(1): 34-38. DOI: 10.3724/SP.J.1238.2011.00034 |
| 作者姓名： | 丁丁 |
| 作者单位： | 1. 云南省农业科学院,花卉研究所,云南,昆明,650205;云南省花卉育种重点实验室,云南,昆明,650205;湖南农业大学,园艺园林学院,湖南,长沙,410128 2. 湖南农业大学,园艺园林学院,湖南,长沙,410128 3. 云南省农业科学院,花卉研究所,云南,昆明,650205;云南省花卉育种重点实验室,云南,昆明,650205 |
| 基金项目： | 云南省科技计划项目，国家科技支撑计划项目，云南省社会发展科技计划项目 |
| 摘 要： | 以百合品种元帅(Acapulco)、卡萨布兰卡(casablanca)的愈伤组织为试验材料,附加不同浓度的枯萎病菌毒素粗提液,用于抗尖孢镰刀菌无性系的离体筛选.结果表明,以体积分数75%的毒索培养基上培养10d作为百合抗尖孢镰刀菌无性系筛选的选择压比较适宜,抗镰刀菌无性系的再生苗生长正常,叶片中过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)活性均比未经筛选的对照株高,经人工接种枯萎病菌鉴定,2个百合品种抗尖孢镰刀菌无性系的再生植株均表现中抗枯萎病. |
| 关 键 词： | 百合 尖孢镰刀菌 无性系 枯萎病 |
| 收稿时间： | 2010-06-11 |
| 修稿时间： | 2010-10-12 |
In vitro selection for lily clonal lines with Fusarium oxysporum f. sp. lilii toxin |
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| dingding. In vitro selection for lily clonal lines with Fusarium oxysporum f. sp. lilii toxin[J]. Journal of Hunan Agricultural University, 2011, 37(1): 34-38. DOI: 10.3724/SP.J.1238.2011.00034 | |
| Authors: | dingding |
| Affiliation: | DING Ding,L(U) Chang-ping,WANG Ji-hua,CUI Guang-fen,WU Xue-wei,ZHANG Yi-ping |
| Abstract: | In order to obtain anti-Fusarium clones of lily, callus of Acapulco and Casablanca were employed as material for the seclection of anti-Fusarium clones by adding various concentrations of toxin in MS medium. The result showed that medium containing 75% of the toxin and 10 d culture can be used as selection pressure for screening lily anti-Fusarium clones and its regeneration plants of disease-resistance had healthy growth. It was found that activity of three enzymes, POD, PPO and SOD, in screened plants was higher than that of control and the screened plants were healthy. Lily anti-Fusarium clones showed a middle level of resistance to lily blight disease by identification of disease-resistance levels. |
| Keywords: | lily Fusarium oxysporum f. sp. lilii clones wilt |
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