猪繁殖候选基因HoxA10的克隆及表达分析

同源异形盒A10基因(Homeobox 10 gene,HoxA10)是Hox基因家族中重要一员,与子宫形态的发生,生育期子宫内膜的周期性形态发育密切相关,是与猪繁殖性状相关的重要候选基因.以长白猪为材料,采用RT-PCR方法,克隆了猪HoxA10基因,并用Real-Time PCR测定该基因在猪各组织器官中的表达.结果表明,从猪子宫组织中克隆获得HoxA10基因cDNA长538 bp,包括1个285 bp的开放阅读框,编码合成94个氨基酸残基,与人和小鼠的HoxA序列同源性分别为98.9%和97.9%;在猪各组织中,前肌是HoxA10基因表达量最高的组织,其次为肾、子宫、后肌、输卵管、大肠、腹脂等组织,在垂体、大脑、小脑、丘脑、卵巢、肺、胃、小肠、背肌、背膘中,HoxA10的表达很低或基本无表达.

Cloning and expression analysis of HoxA10, a candidate gene influencing reproduction traits in pigs

As a key member of Hox gene family, the Homeobox A10 gene (HoxA10) is an important candidate gene influencing reproduction traits in pigs, which plays important roles in embryonic development and cell differentiation. In this paper, HoxA10 gene was cloned from a Landrace pig by RT-PCR, and different tissues from the pig were tested by real-time PCR to determine the tissue-specific expression pattern of HoxA10. Results showed that the cloned HoxA10 cDNA of pig was 538 bp long, and it contained an open reading frame (ORF) of 285 bp encoding a peptide of 94 amino acid residues which showed 98.9% and 97.9% sequence identity to that of human and mouse respectively. In all tested pig tissues, HoxA10 expressed predominantly in forward leg muscle, followed by kidney, uterus, back leg muscle, oviduct, large intestine and abdominal fat. And little or no Expression of HoxA10 was detected in hypothalamus, cerebrum, cerebellum, thalamus, ovary, lung, stomach, small intestine, dorsal muscles and back fat. Key words: pig; HoxA10 gene; cDNA cloning; tissue expression