Adaptive genetic diversity and population structure of the “algarrobo” [<Emphasis Type="Italic">Prosopis chilensis</Emphasis> (Molina) Stuntz] analysed by RAPD and isozyme markers |
| |
Authors: | L I Ferreyra J C Vilardi D S Tosto N B Julio B O Saidman |
| |
Institution: | 1.Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires,Buenos Aires,Argentina;2.Laboratorio de Genética de Poblaciones Aplicada, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires,Buenos Aires,Argentina;3.Instituto de Biotecnología, INTA-Castelar,Buenos Aires,Argentina;4.Cátedra de Genética, Facultad de Ciencias Exactas, Físicas y Naturales,Universidad Nacional de Córdoba,Córdoba,Argentina;5.Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires,Buenos Aires,Argentina |
| |
Abstract: | The “algarrobo” Prosopis chilensis (Molina) Stuntz] is a tree species that represents an important natural resource in arid and semi-arid regions of Argentina.
In this paper, we analysed and compared the variability of 46 RAPD (Random Amplified Polymorphic DNA) loci with previous estimates
obtained from 12 isozyme markers in nine Argentinean populations of P. chilensis representative of the whole range of this species in Argentina. We evaluated the population structure and the existence of
genetic variants associated with environmental variables. Expected heterozygosity (H
e) estimated from RAPD varied significantly among populations and regions. Hierarchical analysis of genetic variability (AMOVA)
showed that most (88.1%) of the total diversity occurs within populations, the component among populations within regions
(9.3%) was intermediate, while the between-region component was the lowest (2.6%). All three variance components were highly
significant. The MDS plot from pair-wise Φ
ST matrix was consistent with the highly significant among-region differentiation indicated by the AMOVA. All 12 variable isozyme
loci and 26 out of 46 RAPD loci showed significant or highly significant association with at least one geographic/climatic
variable according to the stepwise multiple regression analysis. These results imply that the genetic differentiation among
populations is better explained by environmental or biogeographical grounds than by geographical distances, suggesting gametic
disequilibrium with loci responsible for the adaptation to particular environmental conditions. The information from RAPD
markers would provide a relevant criterion to preserve genetic diversity in programmes for conservation and rationale use
of this species. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|