小麦高代重组自交系中HMW-GS基因的分子鉴定

以JY970012群体内11个F8代重组自交系为材料，采用SDS—PAGE技术和AS—PCR技术对HMW—GS组成进行鉴定。结果表明：每个重组自交系在Glu-A1、Glu—B1、Glu—D1位点上HMW—GS组成稳定。F8代，AS—PCR技术在苗期鉴定表明：含有1亚基的株系具有约1500bp的特异带，而含有null亚基的株系没有此带；含有null亚基的株系在约920bp处出现特异带，而1亚基没有。SDS—PAGE和AS—PCR两种技术鉴定结果一致，同时，PCR技术简便、快速、准确、科学，可检测优质亚基基因，为小麦品质选育提供理论依据。

Molecular Identification of HMW-GS in High Gap of Recombinant Inbred Lines(RILs) from Wheat

11 recombinant inbred lines from JY 970012 were analyzed on the compositions of HMW-GS with SDS-PAGE and AS-PCR methods.The result revealed that the compositions of HMW-GS types of the descendants.At seedling stage of F8 gap,using AS-PCR technich to delect HMW glutenin 1 or null at Glu-A 1 locus.The result showed that: there occurred a special band of about 1 500 bp in lines which had 1 subunit,but not in lines which had null subunit.Conversely,RILS which had null subunit occurred a special band of about 920 bp,but PILS which had 1 subunit not occurred the band.The result of PCR identification and of the SDS-PAGE delection are unanimous,at the same time,PCR technich was great helpful to nanlyse good quality,such being simple,fast,accurate and scienlific,so this method can be as served some theoretical basis for quality selection.