农杆菌介导的玉米自交系愈伤组织的转化 |
| |
引用本文: | 权瑞党,尚梅,张举仁.农杆菌介导的玉米自交系愈伤组织的转化[J].山东农业科学,2003(2):3-6. |
| |
作者姓名: | 权瑞党 尚梅 张举仁 |
| |
作者单位: | 山东大学生命科学学院,山东,济南,250100 |
| |
基金项目: | 国家高技术发展计划(2002AA212071)资助. |
| |
摘 要: | 以农杆菌LBA4 4 0 4介导将外源基因导入玉米骨干自交系齐 319、掖 5 15、掖 5 0 2等胚性愈伤组织 ,并由筛选出的抗性愈伤组织再生出可育植株。农杆菌浓度、共培养时间和玉米的基因型显著影响农杆菌介导的玉米愈伤组织的转化率。农杆菌的浓度OD60 0 0 5~ 0 7,共培养时间 3~ 4天时转化率较高。在相同条件下不同基因型的玉米胚性愈伤组织的转化率为 3 3%~ 10 %。
|
关 键 词: | 农杆菌 玉米 愈伤组织 转化 |
文章编号: | 1001-4942(2003)02-0003-04 |
修稿时间: | 2002年12月19 |
Calli transformation of maize inbred via Agrobacterium |
| |
Abstract: | Embryogenic calli of elite maize inbred Qi319, Ye515 and Ye502 were transformed via Agrobacterium tumefaciens , and plantlets were regenerated from resistant calli The concentration of Agrobacterium, duration of co-culture and the genotype of maize could affect the transformation efficiency of calli via Agrobacterium With the concentration of Agrobacterium at OD 600 0 5-0 7, and 3-4 days of co-culture, the transformation efficiency of calli was higher compared with other conditions Under identical conditions, the transformation efficiency of different genotypes of calli was 3 3%-10% |
| |
Keywords: | Agrobacterium maize calli transformation |
本文献已被 维普 万方数据 等数据库收录! |
|